Growth factor-loaded silk fibroin

A silk fibroin and growth factor technology, applied in growth factors/inducing factors, animal/human proteins, carrier binding/immobilized peptides, etc., can solve problems such as prone to sequelae, and achieve the effect of promoting early healing and rapid degradation

Pending Publication Date: 2022-08-05
苏州苏豪生物材料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For lumbar spine injury and cartilage injury, there is currently no better treatment method except surgery, but for surgical treatment, sequelae are also prone to occur, so here we provide a silk fibroin loaded with growth factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0035] A growth factor-loaded silk fibroin, comprising the following preparation steps:

[0036] Step 1: prepare silk fibroin solution;

[0037] Step 2: Adjust the solution in step 1 to pH value to reach the isoelectric point;

[0038] Step 3: Concentrate the mixed solution prepared in step 2;

[0039] Step 4: Polycaprolactone is heated and dissolved and then overlapped and spun to make a polycaprolactone skeleton;

[0040] Step 5: place the polycaprolactone skeleton formed by spinning in the concentrated solution prepared in step 4, and let it stand for 3-8 minutes to obtain a polycaprolactone-silk fibroin blend;

[0041] Step 6: freeze-dry the polycaprolactone-silk fibroin blend prepared in step 5;

[0042] Step 7: put the polycaprolactone-silk fibroin blend prepared in step 6 into an organic solvent until the polycaprolactone is completely dissolved, and then take out the silk fibroin skeleton with several micropores on the surface;

[0043] Step 8: Mix the growth facto...

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PUM

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Abstract

The invention relates to silk fibroin loaded with growth factors, which is characterized in that a polycaprolactone framework is preferentially prepared by using a spinning technology or a 3D printing technology, then the polycaprolactone framework is placed in a silk fibroin solution, and the silk fibroin is attached to the polycaprolactone framework; the silk fibroin gel is prepared from polycaprolactone and silk fibroin, then the polycaprolactone and the silk fibroin are placed in an organic solvent, the polycaprolactone is dissolved, the silk fibroin cannot be dissolved, so that the formed silk fibroin gel is provided with a plurality of micropores, and when the silk fibroin gel with the structure is attached to an injured part, the effect on the skin surface and the effect on the interior of the body are better than those of a traditional medical material.

Description

technical field [0001] The present invention relates to a silk fibroin, in particular to a growth factor-loaded silk fibroin. Background technique [0002] Silk fibroin, also known as silk fibroin, is a kind of natural macromolecular fibrin extracted from silk. Its content accounts for about 70% to 80% of silk. Ala) and serine (Ser) account for more than 80% of the total composition; because silk fibroin itself has good mechanical properties and physical and chemical properties, such as good flexibility and tensile strength, air permeability and moisture permeability, slow release, etc., and Different forms can be obtained through different treatments, such as fibers, solutions, powders, films and gels, etc. Therefore, they have been well used in various fields in recent years, such as the field of biomedicine. [0003] Growth factors are a class of polypeptides that are necessary for regulating the normal growth and metabolism of microorganisms, and that regulate cell grow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/54A61L27/56A61L27/58C07K14/475C07K17/04
CPCC07K17/04C07K14/475A61L27/227A61L27/58A61L27/54A61L27/56A61L2300/414A61L2300/604A61L2400/08
Inventor 李强饶义伟陶伟
Owner 苏州苏豪生物材料科技有限公司
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