Method for tissue culturing and breeding bramble

A technology of tissue culture and raspberry, which is applied in the fields of seedling propagation and plant tissue culture, can solve the problems of labor-intensive, time-consuming, and low survival rate, and achieve the effects of shortening the time for slowing down seedlings, increasing the survival rate, and simplifying the operation process

Inactive Publication Date: 2004-12-01
COTTON RES INST SHANXI ACAD OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0003] The invention solves the problems of long time, labor, time, high cost and low survival rate in the existing raspberry seedling tissue culture propagation method, and provides an improved raspberry seedling tissue culture propagation method

Method used

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Embodiment Construction

[0008] The raspberry seedling tissue culture propagation method comprises carrying out raspberry tissue culture in test tubes, and generates raspberry tissue culture rooted seedlings in test tubes. This part of the technology includes the following steps; raspberry explants (stem tip or axillary buds) first Use Hgcl 2 After sterilizing for 10 minutes, use medium (MS+sucrose 30g / L+agar 6.5g / L+6-BA (6-benzylaminopurine) 0.5mg / L+NAA (naphthaleneacetic acid) 0.20-0.25mg / L) for proliferation Subculture, propagation test-tube plantlets carry out rooting culture with rooting medium (1 / 2MS+sucrose 30g / L+agar 6.5g / L+IBA (indole butyric acid) 0.2mg / L), and the rooted tissue culture seedlings are transplanted.

[0009] Prepare the nursery and apply fertilizers, open the cap of the test tube, let the rooted raspberry seedlings in the test tube be cultivated in the nursery for 1-2 days, then wash off the root medium of the test tube seedlings, and plant them according to a certain plant an...

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Abstract

A tissue culture method for reproducing the saplings of Rubus idaeus includes tissue culturing in test tube to obtain rooted saplings, transplanting them in soil bed, and covering them with disposable paper cup or plastic film for 25-30 days. Its advantages are simple process, short period and high survical rate up to more than 90%.

Description

technical field [0001] The invention relates to a technique for plant seedling propagation by means of tissue culture, in particular to a tissue culture propagation method for raspberry seedlings. Background technique [0002] Raspberry is a high-yield economic forest tree, which needs to be planted in a large area in reality. Therefore, it is necessary to carry out tissue culture and rapid propagation of raspberry seedlings. The existing raspberry seedling tissue culture propagation method is to carry out tissue culture and rapid propagation in the laboratory to form rooted rapid propagation seedlings. The test tube cover for tissue culture is opened, and the seedlings are hardened in the indoor bottle for 2-3 days. In the greenhouse or net room, transplant the test tube seedlings into the substrate in the nutrient pot for more than 30 days, and use a shade net to shade and moisturize. Grow into desired economical seedlings. In the above-mentioned existing method, due to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李燕娥张林水李志民李波吴霞上官小霞杨广东翟小菊
Owner COTTON RES INST SHANXI ACAD OF AGRI SCI
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