Natural active protein and peptide separating and purifying process

Abstract

The present invention is natural active protein and peptide separating and purifying process. The natural material with rich protein is processed through defatting, cleaning, homogenizing, mixing with deionized water inside a bioreactor regulating pH to 5-8, heating to 70-90 deg.c, maintaining and stirring for 10-30 min, quick cooling to 30-50 deg.c and mixing with composite proteinase for enzymolysis to obtain mixture of protein and peptide. Through processing with polyethersulfone superlow protein adsorbing membrane via OMEGA technological process treatment and tangent flow superfiltering to grade according to molecular weight, purifying with ion exchange chromatographic mode, etc. natural active protein and peptide products with different molecular and different molecular structure and obtained.

Description

1. Technical field

[0001] The invention relates to a method for separating and purifying natural active proteins and peptides, and belongs to the field of biological product processing. 2. Background technology

[0002] In recent years, the research on proteins and peptides has progressed rapidly, and a variety of natural bioactive proteins and peptides have been isolated from animals, plants, bacteria, and fungi. Regardless of structure and function, natural bioactive proteins and peptides are the most complex compounds in nature. A large number of studies have confirmed that active proteins and peptides have the effects of promoting immunity, hormones, enzyme inhibitors, antibacterial, and antilipidemia. With the deepening of research, the physiological and pharmacological functions of more biologically active proteins and peptides have been gradually confirmed. However, the separation and purification of natural active proteins and peptides has always been the biggest o...

Examples

Embodiment

[0041] 1. Separation and purification of natural active collagen and peptide

[0042] (1) Use 3% Na for animal skin 2 CO 3 The solution is degreased, rinsed with deionized water for 3 times, and homogenized. Weigh 100 kg of homogenized raw materials and mix them with 150 kg of deionized water, and add them to the biochemical reactor; adjust pH=7, temperature 85°C, stir Keep it for 10 minutes, quickly cool down to 40°C; add 20 grams of compound protease, stir evenly, and then enzymatically hydrolyze to obtain a mixture of protein and peptide. According to the different uses of natural active protein and peptide, use polyethersulfone ultra-low Protein adsorption membrane and tangential flow ultrafiltration technology, the mixture of protein and peptide is classified according to the molecular weight;

[0043] (2) Adopt ion exchange chromatographic separation mode, the conditions of its separation and purification:

[0044] Chromatographic column: anion exchange column, effect...

1. Technical field

[0001] The invention relates to a method for separating and purifying natural active proteins and peptides, and belongs to the field of biological product processing. 2. Background technology

[0002] In recent years, the research on proteins and peptides has progressed rapidly, and a variety of natural bioactive proteins and peptides have been isolated from animals, plants, bacteria, and fungi. Regardless of structure and function, natural bioactive proteins and peptides are the most complex compounds in nature. A large number of studies have confirmed that active proteins and peptides have the effects of promoting immunity, hormones, enzyme inhibitors, antibacterial, and antilipidemia. With the deepening of research, the physiological and pharmacological functions of more biologically active proteins and peptides have been gradually confirmed. However, the separation and purification of natural active proteins and peptides has always been the biggest o...

Examples

Embodiment

[0041] 1. Separation and purification of natural active collagen and peptide

[0042] (1) Use 3% Na for animal skin 2 CO 3 The solution is degreased, rinsed with deionized water for 3 times, and homogenized. Weigh 100 kg of homogenized raw materials and mix them with 150 kg of deionized water, and add them to the biochemical reactor; adjust pH=7, temperature 85°C, stir Keep it for 10 minutes, quickly cool down to 40°C; add 20 grams of compound protease, stir evenly, and then enzymatically hydrolyze to obtain a mixture of protein and peptide. According to the different uses of natural active protein and peptide, use polyethersulfone ultra-low Protein adsorption membrane and tangential flow ultrafiltration technology, the mixture of protein and peptide is classified according to the molecular weight;

[0043] (2) Adopt ion exchange chromatographic separation mode, the conditions of its separation and purification:

[0044] Chromatographic column: anion exchange column, effect...