Natural active protein and peptide separating and purifying process

Abstract

The present invention is natural active protein and peptide separating and purifying process. The natural material with rich protein is processed through defatting, cleaning, homogenizing, mixing with deionized water inside a bioreactor regulating pH to 5-8, heating to 70-90 deg.c, maintaining and stirring for 10-30 min, quick cooling to 30-50 deg.c and mixing with composite proteinase for enzymolysis to obtain mixture of protein and peptide. Through processing with polyethersulfone superlow protein adsorbing membrane via OMEGA technological process treatment and tangent flow superfiltering to grade according to molecular weight, purifying with ion exchange chromatographic mode, etc. natural active protein and peptide products with different molecular and different molecular structure and obtained.

Description

1. Technical field [0001] The invention relates to a method for separating and purifying natural active proteins and peptides, and belongs to the field of biological product processing. 2. Background technology [0002] In recent years, the research on proteins and peptides has progressed rapidly, and a variety of natural bioactive proteins and peptides have been isolated from animals, plants, bacteria, and fungi. Regardless of structure and function, natural bioactive proteins and peptides are the most complex compounds in nature. A large number of studies have confirmed that active proteins and peptides have the effects of promoting immunity, hormones, enzyme inhibitors, antibacterial, and antilipidemia. With the deepening of research, the physiological and pharmacological functions of more biologically active proteins and peptides have been gradually confirmed. However, the separation and purification of natural active proteins and peptides has always been the biggest o...

AI Technical Summary

Problems solved by technology

[0003] 1. Most protein and peptide products are biologically active substances. During the separation and purification process, changes in organic solvents, solution pH, and ionic strength can inactivate proteins;

[0004] 2. Materials containing protein and peptide products are unstable, and protein products are easily degraded by proteases;

[0007] my country is a large agricultural country, and natural raw materials such as animal skins, egg whites, soybeans or corn are quite abundant. They are all rich in protein. As far as the existing application level is concerned, they are still in the initial stage, resulting in a great waste of resources and environmental pollution. Pollution, low economic benefits