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Natural active protein and peptide separating and purifying process

A technology for separation and purification of proteins, which is applied in the field of biological product processing, can solve the problems of resource waste, protein inactivation, and low economic benefits, and achieve the effects of low operating costs, high product recovery rate, and increased activity recovery rate

Inactive Publication Date: 2003-03-19
广州瑞谷生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. Most protein and peptide products are biologically active substances. During the separation and purification process, changes in organic solvents, solution pH, and ionic strength can inactivate proteins;
[0004] 2. Materials containing protein and peptide products are unstable, and protein products are easily degraded by proteases;
As far as the existing application level is concerned, it is still in the initial stage, causing a great waste of resources and environmental pollution, and low economic benefits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0041] 1. Separation and purification of natural active collagen and peptide

[0042] (1) Use 3% Na on the animal skin 2 CO 3 The solution is degreased, rinsed with deionized water for 3 times, and homogenized. Weigh 100 kg of homogenized raw materials and mix them with 150 kg of deionized water, and add them to the biochemical reactor; adjust pH=7, temperature 85°C, stir Keep it for 10 minutes, quickly cool down to 40°C; add 20 grams of compound protease, stir evenly, and then enzymatically hydrolyze to obtain a mixture of protein and peptide. According to the different uses of natural active protein and peptide, use polyethersulfone ultra-low Protein adsorption membrane and tangential flow ultrafiltration technology, the mixture of protein and peptide is classified according to the molecular weight;

[0043] (2) Adopt ion exchange chromatographic separation mode, the conditions of its separation and purification:

[0044] Chromatographic column: anion exchange column, ...

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PUM

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Abstract

The present invention is natural active protein and peptide separating and purifying process. The natural material with rich protein is processed through defatting, cleaning, homogenizing, mixing with deionized water inside a bioreactor regulating pH to 5-8, heating to 70-90 deg.c, maintaining and stirring for 10-30 min, quick cooling to 30-50 deg.c and mixing with composite proteinase for enzymolysis to obtain mixture of protein and peptide. Through processing with polyethersulfone superlow protein adsorbing membrane via OMEGA technological process treatment and tangent flow superfiltering to grade according to molecular weight, purifying with ion exchange chromatographic mode, etc. natural active protein and peptide products with different molecular and different molecular structure and obtained.

Description

1. Technical field [0001] The invention relates to a method for separating and purifying natural active proteins and peptides, and belongs to the field of biological product processing. 2. Background technology [0002] In recent years, the research on proteins and peptides has progressed rapidly, and a variety of natural bioactive proteins and peptides have been isolated from animals, plants, bacteria, and fungi. Regardless of structure and function, natural bioactive proteins and peptides are the most complex compounds in nature. A large number of studies have confirmed that active proteins and peptides have the effects of promoting immunity, hormones, enzyme inhibitors, antibacterial, and antilipidemia. With the deepening of research, the physiological and pharmacological functions of more biologically active proteins and peptides have been gradually confirmed. However, the separation and purification of natural active proteins and peptides has always been the biggest o...

Claims

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Application Information

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IPC IPC(8): C12P21/00
Inventor 谢君张义正张铭让张强徐宁舒子斌
Owner 广州瑞谷生物技术有限公司
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