Wheat fertile activity recovery gene RF6 molecular mark

A fertility restorer gene and molecular marker technology, applied in genetic engineering, plant genetic improvement, DNA/RNA fragments, etc., can solve the problem of uncertain restoration gene homozygous state, heterozygous state, no SSR marker, no tight linkage, etc. problems, to achieve the effect of improving the efficiency of selection and identification, convenient identification, and speeding up utilization

Inactive Publication Date: 2007-04-25
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

At the same time, it was reported that no closely linked SSR markers were found, and it was predicted that the map was not saturated
Currently, molecular markers linked to Rf6 include RFLP, RAPD and ISSR, all of which are dominant markers, that is, it cannot be determined whether the restorer gene exists in a homozygous state or a heterozygous state
No closely linked codominant SSR markers have been reported

Method used

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  • Wheat fertile activity recovery gene RF6 molecular mark

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Embodiment Construction

[0041] N2114 is the 5 generations of selfed progeny of 2114. It was identified by test crosses as a wheat Ae. spp. translocation line carrying the Rf6 gene located on the 6U chromosome translocation segment of A. spp., and its cytoplasm is T-type cytoplasm. The fertility of the translocation line directly reflects the ability of Rf6 to restore fertility. By crossing the donor parent N2114 of Rf6 with the wheat variety or recipient parent, selfing or backcrossing the offspring, and using the molecular markers MAG218 and gwm88 markers to select individual plants containing Rf6, it is possible to select plants containing recovery Male sterile restorer line with Rf6 gene but different genetic background. In the selection process of the restorer line, due to the use of molecular markers, there are no special requirements for the cytoplasm of the maternal parent in the hybridization. In traditional breeding methods, if the cytoplasm is derived from common wheat, it is difficult to know ...

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Abstract

The invention relates to wheat fertility recovery gene Rf6 molecular tag and its obtaining method and belongs to crop breeding and production fields. F2 population arising from wheat T.um-bellulatum translocation line N2114 with susan is analysed in individual genotype and its fertility. Codominant SSR mark MAG218 and dominant mark gwm88 is obtained, which are closely linked to T cell cytoplasm sterility fertility recovery gene Rf6, and can determine the existence of Rf6 and forecast the wheat plant fertility, so can screening the Rf6 plant rapidly.

Description

1. Technical Field [0001] The molecular marker of the wheat fertility restorer gene Rf6 and the method for obtaining the same, provide a co-dominant molecular marker closely linked with the T-type wheat cytoplasmic male sterility restorer gene Rf6, and belong to the field of crop breeding. It is dedicated to the creation, breeding and identification of T-type wheat male sterile restorer line germplasm. It can also be used for cloning restoration genes and transgenic breeding. 2. Technical background [0002] Cytoplasmic male sterility (CMS) is a kind of maternal inheritance. It cannot produce normal and functional pollen but the female reproductive organs are not affected. The fertility restorer gene Rf located in the nucleus can restore its fertility. Studies have shown that fertility restorer genes are generally dominant. CMS and its fertility restoration system play a great role in the production of crop hybrids. On the one hand, it removes the tediousness of manual emasculati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12N15/29C07H21/04A01H1/04C12Q1/68
Inventor 马正强杨郁文
Owner NANJING AGRICULTURAL UNIVERSITY
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