Camptothecin derivative used as anti-tumor agent and preparation method thereof
A technology of drugs and compounds, applied in the field of new derivatives of camptothecin, which can solve the problems of anti-tumor activity and unsatisfactory low toxicity
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[0059] 105mg (0.3mmol) of camptothecin was first dissolved in 15mL of methanol, then 1.2 equivalents of N-(3-propylamino)morpholine was added, and reacted at 75°C for 14 hours. After the system became clear, the reaction mixture was Pour it into 80 mL of diethyl ether, a yellow precipitate precipitated out, filtered off the precipitate, and washed the crude product with 2×2 mL of cold diethyl ether. It can be seen from TLC that the crude product contains unreacted camptothecin starting material. Dissolve the crude product in a small amount of CHCl 3 , further purified with alumina column chromatography (eluent: ethyl acetate:petroleum ether 1:4->1:1) to obtain 106g of pure compound of formula (I), yield 88%, HPLC showed its purity 99.1%.
[0060] IR(KBr)ν: 1620cm -1 (-CO-NH-)
[0061] 1 H NMR: (CDCl 3 , 300MHz) δpm: 1.07 (t, J=7.2Hz, 3H, 18-CH 3 ); 1.76(q, 2H, 19-CH 2 ); 2.25 (m, 1H, 23-CH 2 ), .2.43(m, 1H, 23-CH 2 ); 2.44(m, 4H, 2×25-CH 2 ); 3.41 (m, 2H, 22-CH 2 )...
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