Human monoclonal antibody of human endophloeodal growth factor receptor 2 protein and its preparing process

Abstract

A humanized monoclonal antibody of human endophloeodal growth factor receptor 2 protein is prepared from enternal segment of HER2 / neucell through such steps as culturing the B cells of the person sensitive to HER2; fusing the said B cells with human osteoma cells (karpas 707 H); screening the external segment monoclonal anti body against HER2 cell; and amplification of the said monoclonal antibody. It features that antigen epitope is the external segment polypeptide of HER2 / neu cell for specifically binding the external segment of HER2 / neu cell, and the all gene sequences for syntehsis come from human B lymph cells and human osteoma cells.

Description

Technical field:

[0001] The invention relates to the field of biomedicine, in particular to a fully human monoclonal antibody of human endothelial growth factor receptor 2 protein and a preparation method thereof. Background technique:

[0002] Human endothelial growth factor receptor 2 protein (HER-2 / neu protein) is a single-chain transmembrane glycoprotein encoded by the proto-oncogene HER-2 / neu located on human chromosome 17 q11-22, with a molecular weight of 185kD. Therefore, it is also called P185. HER-2 / neu gene amplification or protein overexpression can often be detected in cells of many malignant tumors such as breast cancer, ovarian cancer, lung cancer, gastric cancer, and colon cancer, so the overexpression of HER-2 / neu protein is related to the occurrence of tumors. When the HER-2 / neu protein is overexpressed on the cell surface, the number of heterodimers formed by the HER-2 / neu receptor increases, and the cell signaling system will be overactivated, resulting ...