Immunological assay reagents and assay method

Abstract

An immunological analyzing reagent comprising a composition containing a biotin-conjugated antigen or antibody against a substance to be analyzed, and a composition containing an avidin-conjugated microparticle, wherein the biotin-conjugated antigen or antibody contains an amount of biotin which does not substantially agglutinate with the avidin-conjugated microparticle in the absence of the substance to be analyzed, is disclosed. Further, an immunological analyzing method comprising the steps of: bringing into contact a sample, a biotin-conjugated antigen or antibody against a substance to be analyzed, and an avidin-conjugated microparticle, the biotin-conjugated antigen or antibody containing an amount of biotin which does not agglutinate with the avidin-conjugated microparticle in the absence of the substance to be analyzed; and detecting the degree of aggregation between the avidin-conjugated microparticle, and an immunocomplex formed from the substance to be analyzed and the biotin-conjugated antigen or antibody, is disclosed. According to the immunological analyzing reagent and analyzing method, a procedure in which an antibody or antigen is carried on a latex particle is not necessary; self-aggregation of latex particles does not occur; a precise measurement of the substance to be analyzed may be carried out; and an excellent reactivity the same as or superior to that obtained by the conventional sensitized latex method may be obtained.

Description

technical field

[0001] The present invention relates to immunoassay reagent and analysis method. In more detail, it relates to an analytical reagent and an analytical method for an agglutination reaction by utilizing an immune reaction. The above-mentioned "analysis" in this specification includes both quantitative or semi-quantitative "measurement" of the amount of the analyte and "detection" of judging the presence or absence of the analyte. Background technique

[0002] As one of the methods for quantitatively analyzing trace components of biological fluids, immunoassay methods using antibodies or antigens of substances to be analyzed are commonly used. As the immunoassay means, the immunocolorimetric method and immunoturbidimetric method for optically measuring the immune complex generated by the antigen-antibody reaction, as well as the radioimmunoassay and enzyme immunoassay using radioactive substances or enzymes as labels, can be used. Immunoassay. In recent years...

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