Microbial prepn for promoting germination of ephedra seed

Abstract

The present invention relates to microbial preparation for promoting germination of ephedra seed. Two kinds of known bacteria, i.e., yellow pseudomonads and Bacillus subtilis, are separated and screened form rhizospheric soil of wild ephedra and through culture in medium and other steps, the microbial preparation for soaking ephedra seed is prepared. When it is used in soaking ephedra seed, the seedling stage can be 4-5 days brought forward and the seedling rate may be 13-19% raised. When it is used for processing ephedra seedling before being transplanted, it can promote the taking root and tillering and transplanted ephedra seedling.

Description

technical field

[0001] The invention relates to a microbial preparation for improving the rapid germination of ephedra seeds Background technique

[0002] Ephedra (Ephedra) is a small perennial herb shrub, which is one of the main medicinal plant resources. It is a well-known traditional medicinal material both at home and abroad. It is mainly distributed in Xinjiang, Inner Mongolia, Ningxia and Gansu in my country. Ephedra is a medicinal plant in arid desert with strong stress resistance. Ephedrine extracted from ephedra is widely used and has high economic value. At present, the demand for ephedrine in the world is increasing at a rate of 30%. Over the years, due to artificial blind logging, the ecological environment has deteriorated, wild ephedra resources have been decreasing year by year, and the raw materials for ephedrine production are relatively insufficient. In recent years, according to the national spirit of promoting the industrializati...

Examples

Embodiment 1

[0023] Strain isolation and culture

[0024] First prepare the soil extract: put 100 grams of ephedra rhizosphere soil samples into 1000 milliliters of tap water to boil, filter for subsequent use;

[0025] Prepare the medium, based on the percentage concentration:

[0026] Nutrient gravy agar: 0.5% peptone, 0.5% sodium chloride, 0.3% beef extract, 1.1% agar, PH7.2;

[0027] PBY medium: beef extract 0.5%, peptone 1.0%, yeast extract 0.5%, sodium chloride 0.5%, glucose 0.5%, agar 1.1%, PH7.0;

[0028] Put the various components in the medium into the spare ephedra rhizosphere soil extract, sterilize, pour the plate, and then use the conventional plate dilution separation method to separate the strains, the culture temperature is 35 ° C, and the culture time is 24 hours , two strains of Pseudomonas xanthomonas and Bacillus subtilis can be obtained;

[0029] Preparation of soaking bacteria solution:

[0030] Shake flask culture medium: 2% corn flour, 2% bean cake flour, 3% br...

Embodiment 2

[0040] Strain isolation and culture

[0041] First prepare the soil extract: put the soil sample of 200 grams of ephedra rhizosphere into 2000 milliliters of tap water and boil, filter for subsequent use;

[0042] Prepare the medium, based on the percentage concentration:

[0043] Nutrient gravy agar: 1.0% peptone, 1.0% sodium chloride, 0.6% beef extract, 2.2% agar, PH7.2;

[0044] PBY medium: beef extract 1.0%, peptone 2.0%, yeast extract 1.0%, sodium chloride 1.0%, glucose 1.0%, agar 2.2%, PH7.0;

[0045] Put the various components in the medium into the spare ephedra rhizosphere soil extract, sterilize, pour the plate, and then use the conventional plate dilution separation method to separate the strains, the culture temperature is 37 ° C, and the culture time is 24 hours , two strains of Pseudomonas xanthomonas and Bacillus subtilis can be obtained;

[0046] Preparation of soaking bacteria solution:

[0047] Shake flask medium: 4% corn flour, 4% bean cake flour, 6% bra...

technical field

[0001] The invention relates to a microbial preparation for improving the rapid germination of ephedra seeds Background technique

[0002] Ephedra (Ephedra) is a small perennial herb shrub, which is one of the main medicinal plant resources. It is a well-known traditional medicinal material both at home and abroad. It is mainly distributed in Xinjiang, Inner Mongolia, Ningxia and Gansu in my country. Ephedra is a medicinal plant in arid desert with strong stress resistance. Ephedrine extracted from ephedra is widely used and has high economic value. At present, the demand for ephedrine in the world is increasing at a rate of 30%. Over the years, due to artificial blind logging, the ecological environment has deteriorated, wild ephedra resources have been decreasing year by year, and the raw materials for ephedrine production are relatively insufficient. In recent years, according to the national spirit of promoting the industrializati...

Examples

Embodiment 1

[0023] Strain isolation and culture

[0024] First prepare the soil extract: put 100 grams of ephedra rhizosphere soil samples into 1000 milliliters of tap water to boil, filter for subsequent use;

[0025] Prepare the medium, based on the percentage concentration:

[0026] Nutrient gravy agar: 0.5% peptone, 0.5% sodium chloride, 0.3% beef extract, 1.1% agar, PH7.2;

[0027] PBY medium: beef extract 0.5%, peptone 1.0%, yeast extract 0.5%, sodium chloride 0.5%, glucose 0.5%, agar 1.1%, PH7.0;

[0028] Put the various components in the medium into the spare ephedra rhizosphere soil extract, sterilize, pour the plate, and then use the conventional plate dilution separation method to separate the strains, the culture temperature is 35 ° C, and the culture time is 24 hours , two strains of Pseudomonas xanthomonas and Bacillus subtilis can be obtained;

[0029] Preparation of soaking bacteria solution:

[0030] Shake flask culture medium: 2% corn flour, 2% bean cake flour, 3% br...

Embodiment 2

[0040] Strain isolation and culture

[0041] First prepare the soil extract: put the soil sample of 200 grams of ephedra rhizosphere into 2000 milliliters of tap water and boil, filter for subsequent use;

[0042] Prepare the medium, based on the percentage concentration:

[0043] Nutrient gravy agar: 1.0% peptone, 1.0% sodium chloride, 0.6% beef extract, 2.2% agar, PH7.2;

[0044] PBY medium: beef extract 1.0%, peptone 2.0%, yeast extract 1.0%, sodium chloride 1.0%, glucose 1.0%, agar 2.2%, PH7.0;

[0045] Put the various components in the medium into the spare ephedra rhizosphere soil extract, sterilize, pour the plate, and then use the conventional plate dilution separation method to separate the strains, the culture temperature is 37 ° C, and the culture time is 24 hours , two strains of Pseudomonas xanthomonas and Bacillus subtilis can be obtained;

[0046] Preparation of soaking bacteria solution:

[0047] Shake flask medium: 4% corn flour, 4% bean cake flour, 6% bra...