Extracting technology for mountain ophiopogon root polysaccharide

An extraction process, the technology of Lie Dong, which is applied in the field of extracting plant polysaccharides, can solve the problems of poor operability and achieve low drying temperature, remarkable effect and good performance

Inactive Publication Date: 2004-06-16
HUBEI UNIV
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  • Abstract
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This method is too random and empirica...

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  • Extracting technology for mountain ophiopogon root polysaccharide
  • Extracting technology for mountain ophiopogon root polysaccharide
  • Extracting technology for mountain ophiopogon root polysaccharide

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Embodiment Construction

[0026] The specific implementation of the present invention will be further described in detail below in conjunction with the examples. The following are described in the order of the process and steps:

[0027] A. First, use high performance liquid chromatography-mass spectrometry to select the medicinal materials of Litium japonicus, and the medicinal materials used in the present invention are Litium japonicus produced in Hubei. Adopt high performance liquid chromatography-mass spectrometry to obtain the total ion current chromatogram, calculate the retention time and peak area of ​​each peak in the figure, and select the relative standard deviation (RSD value) through the methodological investigation of stability, reproducibility and precision. ) less than 5% of the 17 peaks as the characteristic peaks, thus establishing the characteristic fingerprints of Liepedo medicinal material. In the same way, the retention time and area of ​​17 peaks of the medicinal material to be...

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Abstract

The invention discloses a process for extracting liriope spicata lour polysaccharides comprising the steps of, A. using liquid phase chromatogram-mass spectrometer to select liriope spicata lour medicinal material whose root tuber has the similar component and content as the standard medicinal materials, B. producing the selected root tuber into coarse powder, coarse powder displacement using ethanol, C. removing alcohol from slag, extracting through water-bath, D. combining and concentrating the filter liquor, alcohol separating, E. vacuum drying of alcohol separated deposition, chalking in to obtain powder form liriope spicate lour polysaccharide extract, F. measuring the content of liriope spicate lour polysaccharide in the extract through spectrophotometry.

Description

technical field [0001] The invention belongs to a method for extracting plant polysaccharides, in particular to a method for extracting polysaccharides from the Chinese medicinal material Limepogon japonicus. Background technique [0002] The conventional method for extracting plant polysaccharides is to use 95% ethanol (or methanol) to heat and reflux to remove interfering components such as monosaccharides, oligosaccharides, and glycosides, and then add water to extract the polysaccharide components multiple times. [0003] Several scholars at the Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine (see the 59th to 60th pages of Volume 23, Issue 1, published in the Journal of Zhejiang University of Traditional Chinese Medicine in February 1999) believe that: since Ophiopogon japonicus polysaccharides are mainly composed of glucose and fructose, the composition It is relatively simple, using the conventional extraction method to ...

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Application Information

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IPC IPC(8): C08B37/00
Inventor 陈勇李紫蔡敏
Owner HUBEI UNIV
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