Unlock instant, AI-driven research and patent intelligence for your innovation.

Light-stabilized in vivo stain composition and method of manufacture

A dye and molecular technology, applied in the field of thiazide dye biological dyeing compositions, can solve the problems such as clinical observation cannot be carried out normally, the initial composition is uncertain, changes, etc.

Inactive Publication Date: 2004-11-10
ZILA BIOTECHNOLOGY INC
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of this substance, which is generally called "TC" in the prior art, has led to the failure of normal clinical observation, and there are also serious problems in the necessary regulation of the manufacture and sale of this product for human diagnosis
[0003] In addition to the problem of uncertain initial composition, the composition of existing TC and other thiazide biostains can change over time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Light-stabilized in vivo stain composition and method of manufacture
  • Light-stabilized in vivo stain composition and method of manufacture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] This example explains the photoinstability of TC products containing very small amounts of metal ions.

[0017] A sample of the TC product was prepared according to Example 1 of the applicant's US Patent 6,086,852 (herein incorporated by reference) and then further purified by preparative high performance liquid chromatography. The sample had a total metal content of less than 0.2% by weight.

[0018] An aliquot of this purified TC product sample is dark blue. After standing overnight in the laboratory, the solution became colorless when exposed to an incandescent lamp environment.

Embodiment 2

[0020] Using the sample in Example 1, the difference is that about 4% by weight of zinc (in the form of zinc chloride) and 9% of sodium (in the form of sodium chloride) are contained in the solution, and the sample is tested under an incandescent lamp environment. In fact, it is absolutely stable.

Embodiment 3

[0022] Repeat the process of Examples 1 and 2 with methylene blue, sky blue A and sky blue C instead of TC, and similar results can be obtained.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Photochemical demethylation reactions in solutions of thiazine dyes, in which the dye molecules act as both sensitizer and substrate, are reduced by quenching triple-state dye molecules, returning them to the unreactive ground state.

Description

technical field [0001] The present invention relates to photostable thiazine dye biostaining compositions, for example, the chloride toluidine blue ("TC") dye composition disclosed in the applicant's US Patent No. 6,086,852. In another aspect, the invention also relates to processes for the preparation of these compositions. In particular, the present invention relates to photostable toluidine blue chloride ("TC") dye compositions and methods for their preparation. Background technique [0002] According to reports, past workers such as Mashberg (US Patent No. 4,321,251) proposed to use TC for the recognition of abnormal structures in vivo. He adopted the dye product in the prior art. In this dye product, the conformer of TC and the The sum of the N-demethylated and N,N-demethylated derivatives of a conformer is less than 80%, and in the dye product both N-demethylated derivatives of said conformer The alkylated derivatives constituted more than about 20% of the dye compos...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D279/18A61K47/02A61K49/00C09B21/00C09B67/00
CPCA61K49/006A61K47/02A61K49/00
Inventor 道格拉斯·D·伯克特
Owner ZILA BIOTECHNOLOGY INC