Process for separating and extracting lipids general alkaloid from aconites and wolfsbanes

An extraction method, the technology of Aconitum, applied in the field of extraction and separation of lipid alkaloids, can solve the problems of low yield of lipid alkaloids, difficult separation and extraction, incomplete alkaloids, etc.

Inactive Publication Date: 2005-01-26
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

But because lipid alkaloids are difficult to dissolve in acid water, the alkaloids extracted by acid water percolation method is not comprehensive, and the yield of lipid alkaloids is low; Difficult to extract in large quantities; using ethanol to extract alkaloids is more efficient, but ethanol extracts a wide range of components and high impurity content, how to further separate and purify is very important
Therefore, although the research on lipid alkaloids is very urgent, due to the difficulty of separation and extraction, the actual research progress is very slow.

Method used

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  • Process for separating and extracting lipids general alkaloid from aconites and wolfsbanes
  • Process for separating and extracting lipids general alkaloid from aconites and wolfsbanes
  • Process for separating and extracting lipids general alkaloid from aconites and wolfsbanes

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Embodiment 1

[0012] Soak 2,000 grams of raw aconite in 95% ethanol by volume and extract it ultrasonically for 1 hour, cold soak for 48 hours, filter out the ethanol extract, evaporate the ethanol to dryness under reduced pressure at 60°C to obtain ethanol extract, add volume concentration It is 0.04% hydrochloric acid aqueous solution, the addition amount is 10 times the volume of the extract, stir, stand still, filter to obtain a precipitate, dissolve the precipitate in chloroform, and extract twice with a volume ratio concentration of 0.04% hydrochloric acid aqueous solution, each time the amount is the same as that of the chloroform solution of the same volume. Chloroform was evaporated under reduced pressure at 40°C to obtain 9.5 g of crude lipid alkaloids. Use silica gel column, cyclohexane-ethanol 8: 2 system column chromatography, obtain pure lipid alkaloid 4.3 grams (yield rate is 0.21%), use electrospray mass spectrometry to detect, as attached image 3 Shown free of mono- and d...

Embodiment 2

[0014] Soak 5,000 grams of aconite in 95% ethanol by volume for ultrasonic extraction for 2 hours, cold soak for 48 hours, filter out the ethanol extract, evaporate the ethanol to dryness under reduced pressure at 60°C to obtain ethanol extract, add volume specific concentration of 0.04% hydrochloric acid aqueous solution, the addition amount is 8 times of the volume of the extract, stir, stand still, filter to obtain a precipitate, dissolve the precipitate in chloroform, extract 4 times with 0.04% hydrochloric acid aqueous solution, each time the volume is the same as that of the chloroform solution, 40 The chloroform was distilled off under reduced pressure at ℃ to obtain 23 g of crude lipid alkaloids. Use silica gel column, cyclohexane-ethanol 8: 2 system column chromatography, obtain pure lipid alkaloid 13.5 grams (0.27%), use electrospray mass spectrometry to detect, as attached image 3 Shown free of mono- and di-ester alkaloids.

Embodiment 3

[0016] Soak 2000 grams of Aconitum aconitum in 90% ethanol by volume, ultrasonically extract for 3 hours, cold soak for 3 days, and filter out the ethanol extract; use the soaked Aconitum aconitum with a volume concentration of 90% ethanol to repeat under the same conditions Extract twice to obtain the ethanol extract, evaporate the ethanol to dryness under reduced pressure at 60°C to obtain the extract, add sulfuric acid aqueous solution with a volume ratio concentration of 0.3%, and add 6 times the volume of the extract, stir, stand still, and filter to obtain a precipitate , dissolve the precipitate in petroleum ether, use 0.3% sulfuric acid aqueous solution to extract 4 times, each time the volume is the same as the petroleum ether, and distill the petroleum ether under reduced pressure at 40°C to obtain the total alkaloids of crude lipids 19 grams. Use silica gel column, cyclohexane-ethanol 8: 1 system column chromatography to obtain 6.09 grams of pure lipid alkaloids (0....

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Abstract

The invention relates to a process for separating and extracting lipids general alkaloid from aconites and wolfsbanes, wherein the long ester chain, small polarity and property of infusible to water for the lipid alkaloid are utilized for carry out the extraction, the process consists of ethanol cold soaking, acid water treatment, and performing chromatography with silica gel column.

Description

technical field [0001] The invention belongs to a method for extracting and separating lipid alkaloids from aconite and aconitum plants. Background technique [0002] Aconite and Aconitum are commonly used Chinese herbal medicines in traditional Chinese medicine. It has the effects of strengthening the heart, diuresis, analgesia, anesthesia, anti-inflammatory, and improving immunity. Aconitum and Aconitum mainly contain three types of alkaloids: monoester alkaloids, diester alkaloids and lipid alkaloids. Modern pharmacological studies have proved that lipid alkaloids have significant biological activities such as analgesia and anti-inflammation. Compared with diester alkaloids, they are less toxic. application prospects. [0003] In the early days, benzene was often used to extract alkaloids in aconitum and aconite (Wang Yonggao et al., Acta Pharmaceutica: Vol.15(9), 1968, 527-531; -73; Chen Yan et al., Acta Pharmaceutica Sinica: Vol.12(7), 1965, 435-439), although the u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07G5/00
Inventor 刘淑莹许庆轩刘志强宋凤瑞王勇
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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