Biochemical detection liquid for nitrite in urine
A technology for nitrite and biochemical detection, applied in biological testing, analysis through chemical reaction of materials, material inspection products, etc., can solve problems that have not been done before, and achieve the effect of strong versatility and social popularization
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Embodiment 1
[0023] Preparation of urine nitrite biochemical detection solution
[0024] Process requirements:
[0025] Dissolve α-naphthylamine and methanol in the solution of glacial acetic acid until completely dissolved, then dissolve p-aminobenzenesulfonic acid in 400 ml of distilled water > 60°C until completely dissolved, then pour it into the upper liquid after cooling, and then add distilled water to the required amount.
[0026] The obtained product is vermilion solution, pH is 2±0.3, absorbance at 560 nm is 0.03~0.5A, and it is stored in a brown bottle protected from light and sealed at room temperature.
Embodiment 2
[0028] Sensitivity of urine nitrite biochemical detection solution
[0029] g%
[0030] The above experiments show that the sensitivity of the detection solution of the present invention can reach about 0.0002-0.00005%.
Embodiment 3
[0032] urine nitrite test
[0033] Add 1 drop of fresh urine on the glass slide, and then add 1 drop of urine nitrite biochemical detection solution on the urine to observe:
[0034] It is dark cherry red, cherry red, light cherry red, light pink, micro pink, and the traces show that the concentration of nitrous acid is 0.005%, 0.0025%, 0.0003%, 0.0002%, 0.0001%, 0.00005%. All positive results indicated the degree of bacteriuria infection.
[0035] The above color development results are in good agreement with the test results of the classical method.
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