Biochemical detection liquid for nitrite in urine

A technology for nitrite and biochemical detection, applied in biological testing, analysis through chemical reaction of materials, material inspection products, etc., can solve problems that have not been done before, and achieve the effect of strong versatility and social popularization

Inactive Publication Date: 2005-01-26
赵青华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, for bacteriuria self-test, there have been no rele...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of urine nitrite biochemical detection solution

[0024] Process requirements:

[0025] Dissolve α-naphthylamine and methanol in the solution of glacial acetic acid until completely dissolved, then dissolve p-aminobenzenesulfonic acid in 400 ml of distilled water > 60°C until completely dissolved, then pour it into the upper liquid after cooling, and then add distilled water to the required amount.

[0026] The obtained product is vermilion solution, pH is 2±0.3, absorbance at 560 nm is 0.03~0.5A, and it is stored in a brown bottle protected from light and sealed at room temperature.

Embodiment 2

[0028] Sensitivity of urine nitrite biochemical detection solution

[0029] g％

[0030] The above experiments show that the sensitivity of the detection solution of the present invention can reach about 0.0002-0.00005%.

Embodiment 3

[0032] urine nitrite test

[0033] Add 1 drop of fresh urine on the glass slide, and then add 1 drop of urine nitrite biochemical detection solution on the urine to observe:

[0034] It is dark cherry red, cherry red, light cherry red, light pink, micro pink, and the traces show that the concentration of nitrous acid is 0.005%, 0.0025%, 0.0003%, 0.0002%, 0.0001%, 0.00005%. All positive results indicated the degree of bacteriuria infection.

[0035] The above color development results are in good agreement with the test results of the classical method.

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Abstract

This is a biochemistry detection liquid of urine nitrate comprising 0.35-0.45w/v percents sulfanilic acid, 0.2-0.3mgw/v percents alpha-naphthylamine, 20-40v/v percents glacial acetic acid, 1 -2v/v percents carbinol and distilled water. This invention offers biochemistry detection liquid and self-checking method of urine nitrate for the mass scoutting and checking dynamically. Through the recording curve diagram in accordance with series detecting color result one can self-estimate something: 1, warning signal of health and pathogenic factor; 2, forepart detectable rate of bacterial urine; 3, the synchronous tracking monitoring of curative effect during the treating course. The above liquid offer the likelihood of carrying out all this function.

Description

technical field [0001] The invention relates to a urine nitrite-based biochemical detection liquid for urine nitrite and its application Background technique [0002] Bacterial infection of the urinary system is a frequently-occurring disease, especially in married women. It is a chronic disease process, and early detection of bacteriuria is very easy to cure. [0003] The curative effect of the bacterial infection of the urinary system is monitored synchronously during the course of treatment. At present, it is difficult for hospitals to achieve synchronous tracking and dynamic detection of the curative effect of drug treatment. [0004] On the other hand, with the continuous improvement of people's health concerns and self-awareness, it is hoped that they can self-dynamically track, monitor and detect early bacteriuria infections under normal conditions other than clinical conditions such as families, communities, and tourist destinations, so as to r...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/52
Inventor 赵青华
Owner 赵青华
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