Quick extracting method for lotus rhizome tissue total RNA

An extraction method and tissue technology, applied in the field of rapid extraction of total RNA from lotus root tissue, can solve the problems of RNA activity loss, RNA loss, unfavorable RNA solution absorption, etc., and achieve the effect of low extraction cost, good repeatability, and wide applicability
CN1587405AInactive Publication Date: 2005-03-02WUHAN UNIV
0 Cites 13 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
WUHAN UNIV
Publication Date
2005-03-02
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
Patent Text Reader

Abstract

The quick extracting process of lotus rhizome tissue total RNA includes the steps of: compounding test chemical preparation and processing test articles; sampling tender leaf and leaf stalk; extracting, precipitating and centrifuging to obtain total RNA; identifying total RNA with ultraviolet spectrophotometer and formaldehyde denatured agarose gel electrophoresis detection. The obtained data and electrophoresis results show that the lotus rhizome tissue with complete total RNA has high purity and high yield. The present invention has simple test process, convenient and safe operation, accurate and effective result and high repeatability. The method is also suitable for the total RNA extraction of other plant tissue with rich polysaccharide and polyphenol.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field:

[0001] The present invention relates to the molecular biology experiments of lotus root gene cloning, gene function and transgenic lotus root, and more specifically relates to a rapid extraction method of total RNA from lotus root tissue, which is suitable for the extraction of total RNA from lotus root and other plant tissues rich in polysaccharides and phenolic compounds . Background technique:

[0002] Lotus root is an important aquatic economic crop. It is not only edible, but also an important ornamental flower and Chinese herbal medicine. The research on it has attracted widespread attention. As far as the genetics of lotus root is concerned, the traditional research is mainly from the morphological aspect, and the recent research is mainly on the molecular aspect by using the method of molecular biology.

[0003] Plant total RNA extraction technology is a basic experimental technique of plant molecular biology and a necessary prerequisite for plant m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More