Process for preparing tissue chips and apparatus thereof

Abstract

It is organization chip process method and its apparatus which comprises sample device 1 and process device 11 and in detail comprises control pressure bar1, guiding module 3, fixed screw 4, sample needle 5 of needle chip 11, reset pressure spring 6, position bolt 7, guiding bar 9, control bolt 12, needle chip control panel 14, array distribution board 15, L-shape board 16 and guiding bar 17. It is to centrally sample through sample device and then to distribute a group of sample needles 5 orderly on the array distribution board 15, and to inject olefin into all the organic samples through pushdown needle chip control board 14 and it is finished when the olefin is solidified. This apparatus in this invention is in no need of olefin block process and stiletto procedure and accuracy position system.

Description

technical field [0001] The invention belongs to biotechnology, relates to tissue chip technology, in particular to a new preparation method of tissue chip and its device, which is suitable for molecular biology research. Background technique [0002] Biochip (biochip) is a high-throughput and high-tech technology developed in the mid-1990s for molecular biology research. It is another biotechnology revolution with great influence after large-scale integrated circuits ( Watson S J, et al., Biol Psychiatry, 1999). According to different biological materials, biochips can be divided into many kinds, such as gene chips, protein chips, cell chips, microorganism chips, tissue chips, etc. In 1998, Kenonen J et al. (Frantz G D, et al., J Pathol, 2001) first reported tissue chip technology, also called tissue microarray (tissue microarray, TMA). Applying this technology, in situ molecular biology research such as immunohistochemistry and in situ hybridization can be performed on do...

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Problems solved by technology

[0004] There are existing patent documents reporting methods for preparing tissue chips, such as the Chinese invention patent application number: 02139474.1, which records a method for preparing tissue chips, which needs to be melted in a relatively long period of time through a constant temperature water bath with a temperature control switch. The temperature of paraffin is slightly higher than its melting point within the range of 5°C to 10°C, and a negative pressure pump connected to the negative pressure box below the embedding tank is required to form a certain negative pressure at the bottom of the embedding tank, and the structure is relatively complicated. The process cost is high, and it is still repeated spotting one by one

China Invention Patent Application No.: 02113230.5, which records the use of semi-melted paraffin to make tissue chips. This method needs to maintain the semi-melted state of paraffin for a long time, which is inconvenient for positioning during the sampling process, and it is difficult to produce high-density and high-quality tissues. chip

European patent WO 2004 / 000992 and U.S. patent PAT.NO.: 6,103,518 have recorded the improvement of the method focusing on the selection of typical tissue lesion sites, and chip production is still a traditional process, and the production cost is relatively high

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