Reagent for fast diagnosing mammitis of ox, sheep, pig, its preparation process and application

A technology for rapid diagnosis and mastitis, applied in applications, dairy products, and analysis through chemical reactions of materials, etc., can solve problems beyond the purchasing capacity of individual farmers, unknown effects, on-site testing, etc., and achieve result judgment Simple, clear, accurate and fast results

Inactive Publication Date: 2006-06-14
SHANDONG OX LIVESTOCK BREEDING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The price of the instrument ranges from several thousand yuan to tens of thousands of yuan, which is beyond the purchasing power of individual farmers, and its operation requires certain technical and power supply conditions, and it cannot be tested on the spot next to cattle, sheep and pigs, which limits its promotion. ,application
In the 1980s, dairy research institutions in Lanzhou and Beijing imitated CMT and developed their own laboratory diagnostic met

Method used

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  • Reagent for fast diagnosing mammitis of ox, sheep, pig, its preparation process and application
  • Reagent for fast diagnosing mammitis of ox, sheep, pig, its preparation process and application
  • Reagent for fast diagnosing mammitis of ox, sheep, pig, its preparation process and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The following components were weighed in percentage by weight: 2.5% sodium dodecylbenzenesulfonate, 5% sodium hydroxide, 0.5% sodium azide, 6% bromocresol violet, and 86% water.

[0025] Sodium hydroxide and sodium azide are mixed with water according to the stated weight percentage to prepare an aqueous solution, then sodium dodecylsulfonate and bromocresol violet are added according to the stated ratio, stirred and mixed to make it dissolve completely, that is It is prepared as a rapid diagnostic reagent for bovine, sheep and porcine mastitis with light purple solution.

Embodiment 2

[0027]Weigh the following components by weight percentage: 15% sodium dodecylbenzenesulfonate, 6.3% sodium hydroxide, 0.2% sodium azide, 5% bromocresol violet, and 73.5% water.

[0028] Sodium hydroxide and sodium azide are mixed with water according to the stated weight percentage to prepare an aqueous solution, then sodium dodecylsulfonate and bromocresol violet are added according to the stated ratio, stirred and mixed to make it dissolve completely, that is It is prepared as a rapid diagnostic reagent for bovine, sheep and porcine mastitis with light purple solution.

Embodiment 3

[0030] The following components were weighed by weight percentage: 30% sodium dodecylbenzenesulfonate, 10.7% sodium hydroxide, 0.3% sodium azide, 4% bromocresol violet, and 55% water.

[0031] Sodium hydroxide and sodium azide are mixed with water according to the stated weight percentage to prepare an aqueous solution, then sodium dodecylsulfonate and bromocresol violet are added according to the stated ratio, stirred and mixed to make it dissolve completely, that is It is prepared as a rapid diagnostic reagent for bovine, sheep and porcine mastitis with light purple solution.

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PUM

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Abstract

The invention discloses a rapid diagnosis reagent for mastitis of cattle, sheep and pig, and the components and the weight per cent is: Sodium dodecyl benzene sulfonate 2.5%-thirty%, sodium hydroxide 1%-15.5%, alternate sodium nitride 0.05%-0.5%, bromocresol purple 1%-10%, and water 45%-95%. The invention also discloses the preparation method for the reagent that is making up water solution with sodium hydroxide, alternate sodium nitride and adding sodium dodecylsulfate, bromocresol purple according to the ratio, whisking and mixing to make it fully dissolving. The invention has fast speed, low cost, high accuracy, repetitiveness and good stability. It is easy to operate, and has broad application range.

Description

technical field [0001] The invention relates to a diagnostic reagent, in particular to a rapid diagnostic reagent for cattle, sheep and pig mastitis, a preparation method and application thereof. Background technique [0002] Mastitis is the response of mammals (mainly referring to cattle, sheep, pigs, and emphatically referring to cows) to damage to the milk-secreting tissues inside their udders. The invasion of pathogenic microorganisms and the toxins they secrete are the main causes of mastitis. According to research reports, the foreign dairy cow mastitis incidence rate is about 40%-70%, and the domestic dairy cow mastitis incidence rate is about 50%-85% (Zhengzhou City Dairy Cow Mastitis Investigation Group, 1988), wherein the clinical mastitis incidence rate is about 50%-85%. 10%-30%, and the incidence of recessive mastitis is about 40%-70% (Jiang Wucheng, 1993). After the occurrence of mastitis in animals, it will lead to huge losses such as waste of milk, a signific...

Claims

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Application Information

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IPC IPC(8): G01N21/77A01J5/013G01N21/80G01N21/82
Inventor 王长法仲跻峰高运东
Owner SHANDONG OX LIVESTOCK BREEDING CO LTD
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