Process for increasing stability of elastase of lichen spore bacillus ZJUEL31410

A technology of Bacillus licheniformis and elastase, which is applied in the directions of enzyme stabilization, biochemical equipment and methods, enzymes, etc., can solve the problems of carcinogenic effect, safety problems, difficulty in obtaining large-scale application, etc., and achieves improved stability and improved stability. The effect of improving the maximum effect efficiency

Inactive Publication Date: 2006-06-28
ZHEJIANG UNIV
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  • Application Information

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Problems solved by technology

Because bromcyanic acid is used in the chemical modification process, it is a highly toxic substance with carcinogenic effect; it will bring huge safety problems to the actual operation and product application
However, the molecular modification of elastase by new molecular biological methods still faces many technical and cost problems, and it is difficult to obtain large-scale application.

Method used

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Examples

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Effect test

Embodiment Construction

[0031] The method for improving the stability of Bacillus licheniformis ZJUEL31410 elastase: adding bovine serum albumin (PA), gelatin (PB) and hydrated calcium chloride (CaCl 2 .2H 2 O); PA, PB, CaCl 2 .2H 2 The feed ratio of O and the crude enzyme solution is shown in Example 1 to Example 10 in Table 1 respectively, and the one without protective agent is the reference group; the enzyme activity retention rate was measured after being incubated in a water bath at 50°C for 60 minutes, and the specific values ​​are shown in Table 4.

[0032] PA

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Abstract

The invention discloses a method uses to increase Bacillus licheniformis ZJUEL31410 elastase stability. It includes the following steps: adding bovine serum albumin, gelatin, and hydration calcium chloride into CGMCC 1397 lichen bacillus ZJUEL31410 elastase coarse enzyme liquid; and their weight-to-volume ratio with the coarse enzyme liquid are respectively 1-3g/100ml, 1-3g/100ml, and 1-3mmol/L. The method can increase lichen bacillus ZJUEL31410 elastase thermal stability and pH tolerance.

Description

technical field [0001] The invention relates to a biological method for improving and improving the stability of bacillus licheniformis ZJUEL31410 elastase. Background technique [0002] Elastase is a broad-spectrum proteolytic enzyme characterized by decomposing insoluble elastin, which has great economic value and application value. In recent years, elastase is mainly used in industries such as medicine, food and daily chemical industry. Elastase has the functions of treating hyperlipidemia, preventing arteriosclerosis, and improving lipid protein metabolism. [0003] my country is a big silk producing country, and silkworm chrysalis is the main by-product of the silk industry, and about 1 ton of dried chrysalis can be obtained for every 1 ton of raw silk produced. Due to the rich nutritional content of dried pupae, it has high health care value. After separation and extraction, it can be used in food, medicine and other industries, and has extremely high economic value....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96C12N9/66
Inventor 何国庆陈启和徐莹
Owner ZHEJIANG UNIV
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