Osteogenesis promoter

An osteogenesis, enhancer technology, applied to promote bone formation. In this field, it can solve the problems of not knowing the promoting effect of lactoperoxidase on osteogenesis, and achieve the effect of preventing or alleviating osteoporosis, keeping the quality consistent, and promoting bone formation.

Inactive Publication Date: 2006-09-20
SNOW BRAND MILK PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is not known that lactoperoxidase contained in this basic protein has an osteogenesis-promoting effect

Method used

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  • Osteogenesis promoter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A column (5 cm in diameter x 30 cm in height) packed with 400 g of cation exchange resin, sulfonated Chitopearl (manufactured by Fujibo Holdings, Inc.) was thoroughly washed with deionized water, and then, 40 L of unsterilized skim milk was allowed to flow at a flow rate of 25 mL / min. through the column. Then, the column was sufficiently washed with deionized water, and eluted with 0.02M carbonate buffer (pH 7.0) containing 1.5M sodium chloride. Next, the eluted fraction containing lactoperoxidase was adsorbed on an S-Sepharose FF column (manufactured by Amersham Biosciences), and the column was sufficiently washed with deionized water. Equilibrate the column with 10mM phosphate buffer (pH7.0), then elute the adsorbed components with a linear gradient of 0-1M NaCl, and collect the components containing lactoperoxidase. This fraction was then subjected to gel filtration chromatography using HiLoad 16 / 60 Superdex 75 pg (Amersham Biosciences) to yield 11 g of lactoperoxid...

Embodiment 2

[0032]5 mg of the lactoperoxidase obtained in Example 1 was suspended in 10 mL of water, protease trypsin (manufactured by Sigma) was added to a final concentration of 0.01% by weight, and enzyme treatment was performed at 37° C. for 1 hour. Next, the enzyme was inactivated by heat treatment at 90° C. for 5 minutes, and then freeze-dried to obtain 4.1 mg of a lactoperoxidase digestion product. Analysis of the lactoperoxidase digestion product thus obtained by the gel filtration technique revealed that the product had a molecular weight of 10,000 or less.

Embodiment 3

[0044] (preparation of bone formation promoter)

[0045] Add 93.4g hydrated crystal glucose, 5g calcium carbonate, 1g sugar ester, and 0.5g flavoring agent to 100mg of the lactoperoxidase obtained in Example 1, mix, and make the mixture into tablets to obtain the bone formation-promoting formula of the present invention. agent.

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Abstract

It is intended to provide an osteogenesis promoter capable of promoting osteogenesis by promoting the differentiation of osteoblasts, and foods, drinks, drugs or feeds for promoting osteogenesis. Namely, an osteogenesis promoter capable of promoting osteogenesis by promoting the differentation of osteoblasts which comprises, as the active ingredient, lactoperoxidase and / or a digestion product obtained by digesting lactoperoxidase with a protease such as trypsin; and foods, drinks, drugs or feeds for promoting osteogenesis containing lactoperoxidase and / or its digestion product.

Description

technical field [0001] The present invention relates to an agent for promoting bone formation, which contains lactoperoxidase and / or its digested product as an effective ingredient. Furthermore, the present invention relates to a food, beverage, medicament or feed comprising lactoperoxidase and / or its digestion products for promoting bone formation. Background technique [0002] In recent years, various bone diseases such as osteoporosis, fractures, and low back pain have increased significantly along with the aging population. In bone tissue, osteogenesis and bone resorption remain constant. In young people, bone formation and bone resorption maintain a balance, but with age, due to various reasons, this balance is broken and shifts to bone resorption (porosis). Sustaining this state for a long time makes the bone tissue brittle, leading to the occurrence of various bone diseases such as osteoporosis, fractures, and low back pain. Prevention of looseness is considered to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61P19/08A23L1/30A23K1/165A61K35/20A61K38/00A61K38/01A61P19/00A61P19/10A61P43/00
CPCA23V2002/00C12Y111/01007A61K38/44A23K1/1653A23L1/3056A23K20/189A23L33/19A61P19/00A61P19/08A61P19/10A61P43/00A23V2250/5425A23V2200/306
Inventor 元賣睦美松山博昭森田如一芹澤篤川上浩
Owner SNOW BRAND MILK PROD CO LTD
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