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Application of polygonatum cyrtonema Hua. Lectin II protein in medicine preparation for treating or preventing AIDS

A technology of lectin and AIDS, which is applied in the field of Polygonatum lectin II protein, can solve problems such as difficult to design drugs, achieve good medicinal prospects, reduce preparation costs, and facilitate industrial production

Inactive Publication Date: 2006-10-04
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment method has also changed from the traditional single drug therapy to a combination of multiple drugs, but it is still difficult to design a long-term drug against this retrovirus

Method used

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  • Application of polygonatum cyrtonema Hua. Lectin II protein in medicine preparation for treating or preventing AIDS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Preparation of Polygonatum Lectin II Protein

[0016] In this embodiment, the preparation method of the luteinum lectin II protein is as follows:

[0017] 1. Crude product preparation

[0018] Extract sliced ​​Polygonatum tuber with normal saline solution (G / V=1:5) overnight, mash it into a paste with a high-speed tissue grinder, soak it with normal saline solution overnight, filter with gauze, and centrifuge (4°C) , 4500r / min, 30min), add solid ammonium sulfate to the supernatant to reach 30% saturation, place at 4°C overnight, centrifuge (4°C, 4500r / min, 30min) to remove precipitation, continue to add ammonium sulfate to the supernatant to 90%, The precipitate was collected by centrifugation, dissolved in distilled water, dialyzed to remove salt, and freeze-dried to obtain a crude sealogen lectin.

[0019] 2. Affinity column separation and purification

[0020] Take 200 mg of the crude product and dissolve it in 20 ml of normal saline solution, dialyz...

Embodiment 2

[0023] Example 2: Preparation of Polygonatum Lectin II Protein

[0024] In this embodiment, the preparation method of the luteinum lectin II protein is as follows:

[0025] 1. Crude product preparation

[0026] Extract sliced ​​and dried Rhizoma Polygonatum tubers with normal saline solution (G / V=1:5) overnight, mash it into a paste with a high-speed tissue grinder, soak with normal saline solution overnight, filter with gauze, and centrifuge (4°C, 4500r / min, 30min), add solid ammonium sulfate to the supernatant to reach 30% saturation, place at 4°C overnight, centrifuge to remove the precipitate as in the previous method, continue to add ammonium sulfate to the supernatant to 90%, and collect the precipitate by centrifugation. The precipitate was dissolved in distilled water, dialyzed to remove salt, and freeze-dried to obtain the crude sealogen lectin.

[0027] 2. Affinity column separation and purification

[0028] The crude product (200 mg) was dissolved in 20 ml of nor...

Embodiment 3

[0033] Embodiment 3: the inhibitory effect of Polygonatum lectin II protein (PCLII) to human immunodeficiency virus (HIV)

[0034] 1 Experimental materials and methods

[0035] 1.1 Materials

[0036] Galanthus nivalis agglutinin (GNA), Scilla campanulata agglutinin (SCA), orchid plant lectin (Listera Ovta lectin, LOL) samples were provided by the laboratory of Professor Van Damme of the Institute of Plant Protection, University of Leuven Provided; Lycoris radiata Agglutinin (LRA) was provided by the Biomaterials Center of Sichuan University; Polygonatum lectin II (PCL II) protein was prepared by the method described in Example 1 or Example 2.

[0037] HIV strains: HIV-1 and HIV-2 are maintained by the Japan-Canada Medical Research Institute.

[0038] Normal cell lines: CEM (T-cell lymphoma cell line) and TH-4 (mouse nerve cell line) were subcultured by the Japan-Canada Medical Research Institute.

[0039] 1.2 Method

[0040] The assay of anti-HIV activity of Polygonatum lect...

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Abstract

Disclosed is Polygonatum cyrtonema Hua.Lectin II (PCL II), a plant protein isolated and purified from Siberian solomonseal rhizome, which has very strong inhibitory action to human immunodeficiency virus (HIV), thus can be used for preparing medicament for the prevention and treatment of AIDS.

Description

[0001] This application is a divisional application, the application number of the original application is: 200410022354.2, the application date: April 20, 2004, the name of the invention: the application of Polygonatum Lectin II protein in pharmacy. technical field [0002] The present invention relates to the application of Polygonatum cyrtonema Hua., a Chinese herbal medicine, which is a liliaceous plant, and particularly relates to the application of Polygonatum cyrtonema Hua. in pharmacy. Background technique [0003] Lectin is a kind of non-enzyme, non-immune-derived protein or glycoprotein that has sugar-binding specificity and can precipitate sugar complexes widely in nature. Since the first plant lectin was found in castor bean, hundreds of lectins have been discovered and isolated. [0004] Polygonatum cyrtonema Hua., a plant of the Liliaceae family, is a traditional Chinese herbal medicine in my country. Ba...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P31/18
Inventor 鲍锦库吴传芳安洁邓洁何晓佳洪志霞吕鸿周刘超陈放
Owner SICHUAN UNIV
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