Blood smear leucocyte dye liquor and its preparing process

A technology of white blood cells and blood smears, applied in the preparation of test samples, organic dyes, material inspection products, etc., can solve the problems of invisible white blood cells, long staining time, white blood cell count errors, etc., and achieve obvious difference in cytoplasmic color, The effect of shortening the staining time and facilitating image recognition

Inactive Publication Date: 2007-03-07
TECOM SCI CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, when performing manual microscopic observation of blood smears or counting leukocytes by photomicrographs, there are mainly the following problems: (1) Since the number of red blood cells is 500-1000 times that of white blood cells, a large number of red blood cells exist, making the smear must be very accurate. Thin so that the number of white blood cells per field of view is greatly reduced when viewed under a microscope
Moreover, in traditional blood smears, in some cases, the accumulation of red blood cells on the white blood cells will cause the white blood cells to be unrecognizable or even invisible, which will cause a large error in the count of white blood cells
(2) Since the number of white blood cells in each field of view is small, it is necessary to count the number of white blood cells according to many fields of view, which makes it take a long time to count the number of white blood cells for each blood smear
(3) The staining solutions used for routine leukocyte blood smear staining include Swiss staining solution, rapid staining solution, Giemsa staining solution, Swiss-Giemsa staining solution, etc., but the staining time is too long, and each blood smear staining The time is at least more than one minute, and some even as long as more than fifteen minutes, which is not conducive to the analysis of large quantities of blood smears, and is not conducive to the realization of automatic analysis of instruments

Method used

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  • Blood smear leucocyte dye liquor and its preparing process
  • Blood smear leucocyte dye liquor and its preparing process
  • Blood smear leucocyte dye liquor and its preparing process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Get 0.1g dodecyltrimethylammonium bromide, be dissolved in 100ml distilled water earlier, heat, stir, obtain concentration and be the dodecyltrimethylammonium bromide solution of 0.1%, then dilute with distilled water again, obtain A solution with a concentration of 0.001%.

[0033] Take 15ml of Tween-80 and dissolve it in 500ml of methanol to prepare a 3% B solution.

[0034] Take 80ml of solution A and 10ml of solution B and mix them, then add 75ml of Swiss-Giemsa dye solution dropwise, and mix with AB mixed solution to obtain the product of the present invention.

Embodiment 2

[0036] Take 2g of cetyltrimethylammonium bromide, dissolve it in 100ml of distilled water, heat and stir to obtain a 2% cetyltrimethylammonium bromide solution, then dilute with distilled water to obtain a concentration of It is 0.01% A solution.

[0037] Take 20ml of TritonX-100 and dissolve it in 500ml of methanol to make B solution with a concentration of 4%.

[0038] Take 60ml of solution A and 10ml of solution B and mix them, then add 80ml of Swiss-Giemsa dye solution dropwise, and mix with AB mixed solution to obtain the product of the present invention.

Embodiment 3

[0040] Get 2.5g octadecyltrimethylammonium chloride, be dissolved in 100ml distilled water earlier, heat, stir, obtain concentration and be the octadecyltrimethylammonium chloride solution of 2.5%, then dilute with distilled water again, obtain A solution with a concentration of 0.05%.

[0041] Take 10ml of TritonX-80 and dissolve it in 2000ml of methanol to make B solution with a concentration of 0.5%.

[0042] Take 100ml of solution A and 20ml of solution B and mix, then add dropwise 100ml of Swiss dye solution, and mix with AB mixed solution to obtain the product of the present invention.

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Abstract

This invention relates to blood smear leucocyte dye liquid, which is composed of amine salt basic ion surface active agent, polyvinyl pyrrolidon or carbowax and regular leucocyte dye liquid with their constitution proportion as 0.0001%-0.04%,0.0125%-2.5% and 30%-75%. This invention can effectively remove the erythrocyte mixture to properly add the blood thickness to make leucocyte number add within each eye scope.

Description

technical field [0001] The invention relates to leukocyte differential counting, in particular to leukocyte dye solution of blood smear and its preparation method. Background technique [0002] White blood cell differential count refers to the classification of white blood cells in peripheral blood into five categories, namely neutrophils, eosinophils, basophils, lymphocytes and monocytes. They each have their own special physiological functions. According to the color characteristics of various types of white blood cells, they are classified and counted, and the relative ratio is obtained to observe the changes in quantity, shape and quality, which is of auxiliary diagnostic significance for diseases. [0003] Staining leukocytes on blood smears, and then counting them manually or by photomicrograph is one of the ways of counting leukocytes at present. [0004] At present, when performing manual microscopic observation of blood smears or counting leukocytes by photomicrogr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N33/48G01N15/00C09B67/00
Inventor 周洪华黄国韦颜箫
Owner TECOM SCI CORP
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