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Preparation method of heterogenous yolk antibody for preventing and curing duck viral hepatitis

A technology for duck viral hepatitis and egg yolk antibody, which is applied in the field of preparation of heterologous egg yolk antibody, can solve the problems of long detection time, inability of products to enter production and application in time, poor test results and poor repeatability, etc. The effect of originality and rapid and accurate test results

Inactive Publication Date: 2007-03-14
重庆永健生物技术有限责任公司
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  • Summary
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AI Technical Summary

Problems solved by technology

Due to differences in the source, variety, and detection age of animals or duck embryos, the inspection results and repeatability in actual production are poor, and the results of the products produced are good and bad due to differences in detection methods.
At the same time, animal tests or duck embryo neutralization tests are used to measure antibody titers, and the detection time is long, resulting in the production of products that cannot be used in production in time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] 1. Seed virus preparation and propagation

[0015] 1.1 The duck viral hepatitis disease material (liver toxicity) stored in the freezer was made into 1:5 mash with sterilized normal saline, after freezing and thawing twice, the supernatant was collected by centrifugation, treated at 56°C for 1 hour, and green, Treat with streptomycin, filter and sterilize through 0.22um microporous membrane, inoculate the allantoic cavity of 9-11 days old non-immune duck embryos, incubate in a 37°C incubator, discard dead duck embryos within 48 hours, and collect For embryos that died within 48-120 hours, allantoic fluid and amniotic fluid were collected. Dead embryos should have typical pathological changes such as hemorrhage.

[0016] 1.2 Preparation of oil emulsion antigen

[0017] Add 0.1% formaldehyde solution to the harvested allantoic venom, and inactivate it at 37° C. for 48 hours. Shake every 4-6 hours during this period. After the inactivation is completed, prepare 96 part...

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PUM

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Abstract

The present invention is the preparation process of yolk antibody for preventing and treating viral hepatitis of green duck. The present invention prepares the yolk antibody for preventing and treating viral hepatitis of green duck with chicken as one heterogenic animal. That is, after inactivated oil emulsion vaccine of viral hepatitis of green duck is used to immunizing laying fowl, specific immunoglobulin is extracted from the yolk for preventing and treating viral hepatitis of green duck. Clinical test and use shows that the yolk antibody, when used in preventing and treating viral hepatitis of green duck, has high preventing and treating effect, long effective period and no risk of propagating diseases vertically of homological antibody.

Description

technical field [0001] The invention relates to a preparation method of heterologous egg yolk antibody used for preventing and treating duck viral hepatitis. Background technique [0002] Duck viral hepatitis disease is an acute, septic, and contagious infectious disease that mainly affects ducklings. The disease mainly occurs in ducklings at the age of 3-7 days. After the onset, it is often an acute course. The incidence rate can reach 100%, and the mortality rate is as high as 80-100%. Ducks over 10 days old can also be infected, but they often show subclinical or recessive infection after infection, and the mortality rate decreases with increasing age. The disease causes huge economic losses to the duck industry, and is the most harmful disease in the duck industry. [0003] The disease has outbreaks all over the world, and often spreads rapidly. Due to the earlier age of onset of the disease, ducklings without maternal antibodies can develop the disease at the age of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10
Inventor 何洪章
Owner 重庆永健生物技术有限责任公司
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