Reagent kit for detecting 2-diabetes serum mark in Chinese people
A biologically active and molecular technology, applied in the field of detection kits for new serum markers of type 2 diabetes in China, can solve the problems of rare serum markers of diabetes and interference with the separation and identification of low-abundance proteins.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0019] Example 1 Two-dimensional electrophoresis combined with mass spectrometry to analyze the differences in the blood serum of diabetic patients and normal individuals
[0020] express protein
[0021] 1. Collection of samples from type 2 diabetic patients and normal controls in the Chinese Han population
[0022]The peripheral blood samples (10 ml) of the type 2 diabetes group and the normal group used in the present invention mainly come from the Department of Endocrinology, Peking Union Medical College Hospital. Diabetes cases can be sporadic or members of diabetic families (but at this time, only one patient is usually selected in each family, and more than one patient can be selected for members without any blood relationship in the same family); normal controls come from normal people, and their There are no diabetic patients (including type I diabetes) within three generations of the family, and the age and sex of the control group and the affected group are matched...
Embodiment 2
[0040] Example 2 Detecting the expression level of the protein in type 2 diabetes patients and normal human serum by using enzyme-linked immunosorbent assay (ELISA)
[0041] 1) Serum coating Add serum diluted in PBS (1:1000 to 1:10000) to 100 μl / well of the enzyme-labeled well, and coat overnight at 4°C
[0042] 2) Standard protein coating Add standard protein serially diluted in PBS to 100 μl / well of the enzyme-labeled well and coat overnight at 4°C
[0043] 3) Blocking Dissolve skimmed milk powder in PBS to prepare 5% blocking solution, discard the coating solution, add blocking solution at 200 μl / well, 37°C, 2h. Pour off the blocking solution, and if not used immediately, dry it in an ultra-clean bench, seal it with tape, and store it at 4°C.
[0044] 4) Add the diluted anti-apolipoprotein A-I antibody (purified IgG, serum, ascites, etc.) to the enzyme-labeled well, 100 μl / well, overnight at 4°C, or 3-4 hours at 37°C.
[0045] 5) Pour off the primary antibody, wash the we...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com