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Lamiophlomis rotata medicine material, intermediate and its injection liquid finger-print atlas quality testing method

A detection method and fingerprint technology are applied in the quality detection of fingerprints of intermediates and their injections, and in the field of unique medicinal materials, which can solve the problems of not reflecting fingerprint characteristics and the like.

Inactive Publication Date: 2010-05-12
SICHUAN HENGKANG DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In No. 200410083678.7 patent application, although the relevant technical content of the unique fingerprint is also involved, this patent application introduces macroporous adsorption resin for pretreatment in the preparation process of medicinal materials, intermediates and injection test samples, which may affect the The final result causes at least two influences: one is that the macroporous adsorption resin will inevitably introduce new substances in the process of adsorption and elution; the other is that the macroporous adsorption resin will supply Adsorption of related substances in the test sample
Can not reflect the real fingerprint characteristics

Method used

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  • Lamiophlomis rotata medicine material, intermediate and its injection liquid finger-print atlas quality testing method
  • Lamiophlomis rotata medicine material, intermediate and its injection liquid finger-print atlas quality testing method
  • Lamiophlomis rotata medicine material, intermediate and its injection liquid finger-print atlas quality testing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0121] Embodiment 1: The method for detecting the quality of the fingerprint spectrum of the unique medicinal material

[0122] Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; flow rate: 1ml / min; detection wavelength: 254nm; column temperature: 30°C; analysis time: 1 hour; gradient elution:

[0123] gradient elution

[0124]

[0125] Preparation of the reference substance solution: take an appropriate amount of quercetin reference substance, add methanol to make a 40 μg / ml solution, and use it as the reference substance solution;

[0126] Preparation of the test solution: Take 5g of medicinal material powder that has passed through a sieve with an inner diameter of 5mm after crushing, put it in a flask, add 100ml of water, and extract by reflux for 2 hours, filter, evaporate the water to dryness, add an appropriate amount of methanol to the residue to dissolve, and centrifuge at 1000r / min , the supernatant was transf...

Embodiment 2

[0129] Embodiment 2: the fingerprint spectrum quality detection method of unique intermediate

[0130] Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; flow rate: 1ml / min; detection wavelength: 254nm; column temperature: 30°C; analysis time: 1 hour; gradient elution:

[0131] gradient elution

[0132]

[0133] Preparation of the reference substance solution: take an appropriate amount of quercetin reference substance, add methanol to make a 40 μg / ml solution, and use it as the reference substance solution;

[0134] Preparation of the test solution: Take 1ml of Duyiwei injection intermediate, put it in a 10ml volumetric bottle, add 1ml of the reference solution precisely, dilute with methanol to the mark, shake well, filter with a 0.45μm microporous membrane, and take the filtrate , that is;

[0135] Assay method: draw 10 μl of the reference substance solution and the test solution, inject them into the liquid chromat...

Embodiment 3

[0137] Embodiment 3: The fingerprint spectrum quality detection method of Duyiwei Injection

[0138] Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; flow rate: 1ml / min; detection wavelength: 254nm; column temperature: 30°C; analysis time: 1 hour; gradient elution:

[0139] gradient elution

[0140]

[0141] Preparation of the reference substance solution: take an appropriate amount of quercetin reference substance, add methanol to make a 40 μg / ml solution, and use it as the reference substance solution;

[0142] Preparation of the test solution: Take 1ml of Duyiwei Injection, put it in a 10ml volumetric flask, add 1ml of the reference substance solution, dilute with methanol to the mark, shake well, filter with a 0.45μm microporous membrane, take the filtrate, and get ready;

[0143] Assay method: draw 10 μl each of the reference substance solution and the test solution, inject it into the liquid chromatograph, and m...

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Abstract

A fingerprint method for testing the quality of common lamiophlomis, its intermediate and its injection includes such steps as preparing the reference solution from quercetin, preparing three solutions of common lamiophlomis, its intermediate and its injection, filling the reference solution and one solution to be tested into liquid-phase chromatograph, and testing the similarity between their fingerprints, which must be greater than 0.90.

Description

technical field [0001] The invention relates to a quality detection method of Chinese medicinal materials, intermediates and injections thereof, in particular to a fingerprint quality detection method of unique medicinal materials, intermediates and injections thereof. Background technique [0002] The main chemical components in Duyiwei mainly include flavonoids, iridoids and some fatty acids. The flavonoids mainly include luteolin, luteolin-7-o-glucoside, quercetin, quercetin-3-o-arabinoside, apigenin and other components. At present, the quality standards of traditional Chinese medicine are not standardized and perfect, and the means of quality analysis and evaluation are still very backward, which cannot accurately reflect the quality status of existing Chinese medicinal materials and finished products. The fingerprint of traditional Chinese medicine is based on the concept of fingerprint identification in forensic medicine. After proper processing, certain analytical m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02A61K36/53A61K125/00
Inventor 阙文彬李育飞
Owner SICHUAN HENGKANG DEV
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