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Distant crossing method of bamboo and rice

A technology of distant hybridization and bamboo, which is applied in the field of rice hybridization, can solve the problems of hybridization of bamboo and rice that have not been seen, and achieve high economic value and application value, good plant shape, and stable traits

Inactive Publication Date: 2007-06-06
钟章美 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] There are many varieties of hybrid rice. At present, there are no related reports on the use of bamboo and rice hybrids.

Method used

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  • Distant crossing method of bamboo and rice
  • Distant crossing method of bamboo and rice

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Experimental program
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Effect test

Embodiment 1

[0013] Hybrid breeding of embodiment 1 bamboo rice 966 and bamboo rice 989

[0014] The method for bamboo and rice distant hybridization of the present invention, its process is referred to as shown in Fig. 1, implements the method of the present invention to be successful, has solved three major technical difficulties: (1) solved pollination and fertilization technology; (2) solved (3) solve the problem of long breeding cycle to prevent the loss of bamboo genes. Below in conjunction with the hybridization selection process of bamboo rice 966 and bamboo rice 989, each step of the inventive method is described in detail as follows:

[0015] 1. Grasp the flowering habits of Bambusa vulgaris, select the female parent and the appropriate killing temperature

[0016] Through several years of observation, we have mastered the flowering law of bamboo, that is, the flowering season is from March to June every year, and the blooming period is from April to May; under normal weather co...

Embodiment 2

[0038] Genetic Testing

[0039] Detection method: Using the Southern hybridization method, the japonica rice variety "Nipponbare" and the indica rice variety "93-11" were used as negative controls, and the original male parent of bamboo rice "Qingpizhu" was used as a positive control to extract "Nipponbare" and "93-11" respectively. 11", "Zhudao 966", "Zhudao 989" and "Qingpizhu", the five DNA samples were completely digested with EcoR I and HindIII, respectively, and electrophoresis, membrane transfer and cross-linking were performed after digestion , using the genome DNA of "Nipponbare" and "93-11" that were completely digested for blocking (pre-hybridization), using the isotope-labeled genome DNA of "Qingpi Bamboo" that was completely digested for hybridization as a probe, and finally washing the membrane and pressing film, washing film.

[0040] Test results: The results of Southern hybridization are shown in Figure 2. It can be seen from Figure 2 that the signal of the p...

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Abstract

The present invention discloses distant crossing method of bamboo and rice, belongs to the field of rice hybridizing technology, and aims at transferring excellent gene of heterogeneous plant to rice to expand and enrich rice gene bank, create new germplasm source and culturing new rice breed. The method includes the following steps: 1. hybridizing Raoke F4 and green bark bamboo to obtain bamboo rice; 2. obtaining the tillering seedling of the first generation of bamboo rice, treating with colchicina solution and cultivating to obtain second generation bamboo rice F2 seed; 3. hybridizing bamboo rice F2 with Baomei F3 to obtain bamboo Baomei F3; 4. hybridizing bamboo rice F2 with Haikechuan rice to obtain bamboo Kechuan F2 and hybridizing bamboo Kechuan F2 with Guichao 2 to obtain Zhuzazao rice; and 5. hybridizing bamboo Baomei F3 with Zhuzazao rice to obtain Zhudao 966 rice and Zhudao 989 rice.

Description

technical field [0001] The invention relates to rice hybridization technology, in particular to a method for distant hybridization of bamboo and rice. Background technique [0002] There are many varieties of hybrid rice, but at present, there is no relevant report utilizing bamboo and rice hybrid. Contents of the invention [0003] The technical problem to be solved in the present invention is to transfer good genes of heterologous plants into rice to expand and enrich the rice gene pool, create new quality sources, and cultivate new varieties to provide a method for distant hybridization of bamboo and rice. [0004] The technical scheme of the present invention is: a method for distant hybridization of bamboo and rice, comprising the following steps: (1) adopting Raopingai and Keqing No. 3 crossbreeding strain Raoke F4 to hybridize Bambusa vulgaris to obtain bamboo Paddy; (2) the first generation of bamboo and rice hybrids cut off the aerial parts, the clover roots surv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H5/00
Inventor 钟章美黄森伦
Owner 钟章美
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