Bioactivation of particles

a technology of bioactivation and particles, applied in the field of microparticles and/or nanoparticles, can solve the problems of difficult simultaneous or even non-simultaneous detection or discrimination between the presence of a number, and the limitation of the number of different color organic dye molecules which may be utilized simultaneously or sequentially,

Inactive Publication Date: 2012-08-16
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The use of bioactivation peptides enables the creation of stable, biocompatible particles that can be used in various biological applications, including simultaneous imaging by multiple modalities and prolonged exposure to light, while maintaining photostability and solubility in aqueous environments.

Problems solved by technology

There are a number of problems with such an analytical system.
As a result, there is a severe limitation on the number of different color organic dye molecules which may be utilized simultaneously or sequentially in an analysis since it is difficult to either simultaneously or even non-simultaneously detect or discriminate between the presence of a number of different detectable substances due to the broad spectrum emissions and emission tails of the labeling molecules.
Another problem is that most dye molecules have a relatively narrow absorption spectrum, thus requiring either multiple excitation beams used either in tandem or sequentially for multiple wavelength probes, or else a broad spectrum excitation source which is sequentially used with different filters for sequential excitation of a series of probes respectively excited at different wavelengths.
Another problem frequently encountered with existing dye molecule labels is that of photostability.
In addition, the probe tools used for the study of systems by electron microscopy techniques are completely different from the probes used for study by fluorescence.
Thus, it is not possible to label a material with a single type of probe for both electron microscopy and for fluorescence.
This results in highly hydrophobic particles, poorly soluble in aqueous environments.

Method used

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  • Bioactivation of particles
  • Bioactivation of particles
  • Bioactivation of particles

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Embodiment Construction

[0021]The present invention generally involves converting particles that are biologically non-functional into bioactivated particles that have one or more functional characteristics that are necessary to make the particles useful in biological systems. This is accomplished by attaching bioactivation peptides to the surface of the particles. These specialized peptides are capable of imparting one or more biologically important functions to the particles. As will be discussed in detail below, the bioactivation peptides of the present invention effectively eliminate the need for conventional linking agents that have been used in the past to connect biologically functional groups to particle surfaces. In addition, the use of bioactivation peptides to impart biological function(s) to particles is extremely versatile and relatively simple. It has wide applications to any type of biological system where particles having specific biological functions are required.

[0022]The term “bioactivate...

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Abstract

Particles are bioactivated by attaching bioactivation peptides to the particle surface. The bioactivation peptides are peptide-based compounds that impart one or more biologically important functions to the particles. Each bioactivation peptide includes a molecular or surface recognition part that binds with the surface of the particle and one or more functional parts. The surface recognition part includes an amino-end and a carboxy-end and is composed of one or more hydrophobic spacers and one or more binding clusters. The functional part(s) is attached to the surface recognition part at the amino-end and / or said carboxy-end.

Description

[0001]This application is a Continuation of U.S. application Ser. No. 10 / 513,567, filed Nov. 3, 2004, which is a National Stage of International Application No. PCT / US2003 / 014401, filed May 7, 2003, which claims the benefit of U.S. Provisional Application No. 60 / 378,720, filed May 7, 2002. Application Ser. No. 10 / 513,567, International Application No. PCT / US2003 / 014401, and U.S. Provisional Application No. 60 / 378,720 are hereby incorporated by reference in their entirety. Other patent applications, patents, and other documents referred to in this application are hereby incorporated by reference in their entirety. This invention was made with Government support of Grant Nos. EB000312 and RR014891 awarded by the National Institutes of Health and Grant No. DE-AC03-76SF00098 awarded by the Department of Energy. The government has certain rights to this invention.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]The present invention relates generally to microparticles and / ...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): C07K14/00A61K38/00A61K47/48B82Y99/00
CPCA61K49/0056A61K49/0067Y10T428/2982B82Y10/00B82Y30/00B82Y5/00
InventorPINAUD, FABIENKING, DAVIDWEISS, SHIMON
OwnerRGT UNIV OF CALIFORNIA