Laser Induced Collagen Crosslinking in Tissue

Inactive Publication Date: 2018-07-12
THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0016]In embodiments, the laser system and the regime of controlling the output of the system irradiates connective tissue such that the wavelength of the laser is not absorbed by the targeted tissue itself, but rather by water in and around the tissue. Thus, optical breakdown of the tissue is avoided, but the laser is controlled to cause ionization of the water molecules, which in turn generates f

Problems solved by technology

Further, the cation radical of water, H2O+, is rather unstable and reacts w

Method used

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  • Laser Induced Collagen Crosslinking in Tissue
  • Laser Induced Collagen Crosslinking in Tissue
  • Laser Induced Collagen Crosslinking in Tissue

Examples

Experimental program
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Example

Example 1: Use of Femtosecond Laser to Cross-Link Porcine Cornea

[0080]This Example illustrates the effect of femtosecond laser irradiation to a porcine cornea.

[0081]Porcine eyes were obtained from a commercial supplier (Animal Technologies, Tyler, Tex.). The eyes were harvested and frozen within 3 hours after slaughter. The eyes were carefully thawed immediately before the example was performed. The globe was mounted onto the metal ring and fixed with cyanoacrylate, such that the cornea was placed at the center and left exposed after the mounting excess tissue was removed. Subsequently, the upper surface of cornea was moistened with phosphate buffered saline (PBS) solution and a coverslip was placed on top of the specimen to reduce light scattering from the laser. Placement of the coverslip also ensured the flatness of the top surface of the cornea. The metal ring with cornea was fixed onto a 3-axis motorized translation stage using a custom made holder.

[0082]A Nd:Glass femtosecond ...

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Abstract

The presently disclosed subject matter provides techniques for inducing collagen cross-linking in human tissue, such as cartilage or cornea, without using a photosensitizer (e.g., riboflavin), by inducing ionization of the water contained in the tissue to produce free radicals that induce chemical cross-linking in the human tissue. In an embodiment, a femtosecond laser operates at sufficiently low laser pulse energy to avoid optical breakdown of tissue. In an embodiment, the femtosecond laser operates in the infrared frequency range.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of International Application No. PCT / US2016 / 058353 filed Oct. 23, 2016, which claims priority to U.S. Provisional Application No. 62 / 245,805 filed Oct. 23, 2015, U.S. Provisional Application No. 62 / 358,035 filed Jul. 3, 2016, and U.S. Provisional Application No. 62 / 380,713 filed on Aug. 29, 2016. The entire contents of each of the above applications is hereby expressly incorporated by reference.BACKGROUND[0002]Collagen is an abundant protein in animals. The mechanical properties and structural stability of collagen based tissues, such as cartilage, tendons, ligaments, or corneal stroma, can be influenced by increasing collagen cross-links (CXL), in the form of intra or inter molecule chemical bonds. CXL are naturally formed in tissues, but inducing new or additional CXL can be beneficial. Strength and the ability to bend under tension are two characteristics of collagen, which is the significant componen...

Claims

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Application Information

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IPC IPC(8): A61F9/008A61B18/20A61B18/26
CPCA61F9/008A61B18/203A61B18/26A61F2009/00872A61F2009/00897A61F2009/00851A61F2009/00882A61B2018/20359A61B2018/20553A61B2018/205545A61B2018/00791A61B2018/263A61N1/44A61B2018/00785A61N5/067A61B90/361A61B18/042A61B2018/00702A61N5/062
Inventor VUKELIC, SINISATROKEL, STEPHEN
Owner THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK
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