Vacuum drying production technology of high-activity bifidobacterium bacterium powder and application

Abstract

The invention discloses a vacuum drying production technology of high-activity bifidobacterium bacterium powder and application and belongs to the technical field of foods. According to the vacuum drying production technology, non-water-soluble dietary fibers are used as a solid-phase carrier and fermentation is carried out to produce a bifidobacterium biological film thallus; after the fermentation, bacterium mud is collected and is mixed with a drying protection agent; a mixture is dried in vacuum to prepare the bifidobacterium bacterium powder. According to the method disclosed by the invention, the vacuum drying survival rate of bifidobacterium can reach 50 percent or more at maximum, the viable count of the bacterium powder reach 1 to 5*10<10>cfu / g and the vacuum drying survival rateof the bifidobacterium is improved by 10 or 10 times or more when being compared with that of bifidobacterium with a non-biological-film state. According to the vacuum drying production technology disclosed by the invention, the key common technical problems in a development process of the high-activity bifidobacterium bacterium powder are solved, and the prepared bifidobacterium bacterium powdercan be applied to product forms including a fermentation agent, solid beverage and the like.

Description

technical field

[0001] The invention relates to a vacuum drying production process and application of highly active bifidobacteria powder, belonging to the technical field of food. Background technique

[0002] Bifidobacterium is an inherent flora in the intestines of humans and animals, and plays an important role in the stability of intestinal microecology and the regulation of host physiological functions. Therefore, since the 1980s, bifidobacteria have been widely used in the fermented dairy industry, and in food and dietary supplements, and have broad market prospects in the modern food industry. Common live bifidobacteria products on the market include: yogurt, bifidobacterium tablets, bifidobacterium chocolate, etc. Most of these products are made from bifidobacterium powder. At present, most of the commercially available bifidobacteria powders are produced by freeze-drying. Although freeze-drying can obtain bifidobacteria powders with a high survival rate, there are...

Examples

Embodiment 1

[0026] Embodiment 1: the vacuum drying of pseudo small chain bifidobacterium

[0027] (1) Preparation of Bifidobacterium film fermentation medium:

[0028] Preparation of Pseudomonas bifidobacterium film fermentation medium: yeast powder 5g / L, anhydrous sodium acetate 5g / L, beef extract 10g / L, anhydrous glucose 20g / L, fish meal peptone 10g / L, magnesium sulfate heptahydrate 0.5g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L, Tween -801g / L, soybean powder 10g / L.

[0029] (2) Fermentation preparation of bifidobacterium bacterium film: bifidobacteria are activated for 3 generations, according to the inoculation ratio of 3-5%, transfer in the 10L fermenter, continuously feed nitrogen, adjust the rotating speed, 37 degrees of constant pH ( pH=6) for 24 hours.

[0030] (3) Collection of bifidobacterium pellicles: Centrifuge the bifidobacteria pellicle fermentation liqu...

Embodiment 2

[0037] According to the operation steps of Example 1, the difference is that oat bran powder is used instead of soybean powder to carry out bacterial film fermentation, and the vacuum drying results show that the vacuum drying survival rate of the bacterial film formed by using oat bran powder as a solid medium is 47%. Significantly higher than the vacuum drying survival rate of bacteria in the non-bacteria film state. The bacteria powder was stored at 25 degrees for 12 weeks, and the survival rate was 49%.

Embodiment 3

[0039]According to the operation steps of Example 1, the difference is that the desiccation protection agent is replaced, and the formula of the desiccation protection agent is: 100g / L skimmed milk powder, 30g / L stachyose, 30g / L sucrose, 0.5g / L vitamin C, and the pH is 6.5. The vacuum-drying survival rate of pseudosmall-chain bifidobacterium formed by biofilm is 45%, which is significantly higher than the vacuum-dried survival rate of bacteria in non-bacteria film state. The bacteria powder was stored at 25 degrees for 12 weeks, and the survival rate was 43%.

technical field

[0001] The invention relates to a vacuum drying production process and application of highly active bifidobacteria powder, belonging to the technical field of food. Background technique

[0002] Bifidobacterium is an inherent flora in the intestines of humans and animals, and plays an important role in the stability of intestinal microecology and the regulation of host physiological functions. Therefore, since the 1980s, bifidobacteria have been widely used in the fermented dairy industry, and in food and dietary supplements, and have broad market prospects in the modern food industry. Common live bifidobacteria products on the market include: yogurt, bifidobacterium tablets, bifidobacterium chocolate, etc. Most of these products are made from bifidobacterium powder. At present, most of the commercially available bifidobacteria powders are produced by freeze-drying. Although freeze-drying can obtain bifidobacteria powders with a high survival rate, there are...

Examples

Embodiment 1

[0026] Embodiment 1: the vacuum drying of pseudo small chain bifidobacterium

[0027] (1) Preparation of Bifidobacterium film fermentation medium:

[0028] Preparation of Pseudomonas bifidobacterium film fermentation medium: yeast powder 5g / L, anhydrous sodium acetate 5g / L, beef extract 10g / L, anhydrous glucose 20g / L, fish meal peptone 10g / L, magnesium sulfate heptahydrate 0.5g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L, Tween -801g / L, soybean powder 10g / L.

[0029] (2) Fermentation preparation of bifidobacterium bacterium film: bifidobacteria are activated for 3 generations, according to the inoculation ratio of 3-5%, transfer in the 10L fermenter, continuously feed nitrogen, adjust the rotating speed, 37 degrees of constant pH ( pH=6) for 24 hours.

[0030] (3) Collection of bifidobacterium pellicles: Centrifuge the bifidobacteria pellicle fermentation liqu...

Embodiment 2

[0037] According to the operation steps of Example 1, the difference is that oat bran powder is used instead of soybean powder to carry out bacterial film fermentation, and the vacuum drying results show that the vacuum drying survival rate of the bacterial film formed by using oat bran powder as a solid medium is 47%. Significantly higher than the vacuum drying survival rate of bacteria in the non-bacteria film state. The bacteria powder was stored at 25 degrees for 12 weeks, and the survival rate was 49%.

Embodiment 3

[0039]According to the operation steps of Example 1, the difference is that the desiccation protection agent is replaced, and the formula of the desiccation protection agent is: 100g / L skimmed milk powder, 30g / L stachyose, 30g / L sucrose, 0.5g / L vitamin C, and the pH is 6.5. The vacuum-drying survival rate of pseudosmall-chain bifidobacterium formed by biofilm is 45%, which is significantly higher than the vacuum-dried survival rate of bacteria in non-bacteria film state. The bacteria powder was stored at 25 degrees for 12 weeks, and the survival rate was 43%.