The invention relates to the field of veterinary medicine, in particular to a tilmicosin and ceftiofur injection and preparing equipment and process. The tilmicosin and ceftiofur injection contains tilmicosin, ceftiofur, aluminum stearate, vitamin C and soybean oil; the preparing equipment comprises a soybean oil storing tank, a filtering device, a mixing and dissolving device, a dry heat sterilizing device, a cooling device, a heating device, a diluting device, a mixing and stirring device, a high-pressure homogenizing device, a stirring and subpackaging device and sterilizing device; the preparing process includes the steps of preparation of sterilizing soybean oil, preparation of aluminum stearate factice of 10%, dilution, mixing and stirring, homogenizing and mixing, stirring and subpackaging and sterilizing. Through the specific preparing equipment and the excellent preparing process, tilmicosin and ceftiofur are prepared to be the oil suspension injection, so that ceftiofur and tilmicosin can be combined for administration to treat respiratory infectious diseases and systemic sepsis caused by mycoplasma secondary infection gram-positive bacteria and gram-negative bacteria infection.