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This invention belongs to the field of hop propagation technology, specifically relating to a method for propagating hops by rock wool cuttings in plug trays. The method for propagating hops by rock wool cuttings in plug trays includes the following steps: (1) Preparing cuttings: Selecting hop mother plants and cutting semi-lignified branches as cuttings; (2) Treating cuttings: After cutting the cuttings, soaking the base in a plant growth regulator; (3) Cutting operation: Dividing the rock wool into small pieces, placing them in plug trays, completely soaking the rock wool in a probiotic solution, and inserting the cuttings into the rock wool; (4) Post-cutting management: Placing the plug trays after cutting in a greenhouse or polytunnel, cultivating for 45-50 days after cutting, and then transplanting. The propagation method of this invention can improve the rooting rate of hops, while the root system of the cuttings is more developed, the plants grow better, and the survival rate after transplanting is higher.