The invention relates to an assessment test method and a device of a timber architecture room environment. The assessment test method includes that physical indexes of the timber architecture room environment and physiological indexes of a testee in the timber architecture room environment are synchronously tested, and physical index data and physiological index data are obtained, wherein the physical indexes include noise, lighting, a temperature and humidity, and the physiological indexes include electrocardio, a heart rate, respiration, a skin temperature, skin conductivity and surface myoelectricity of the testee; and statistic analysis is carried out on the physical index data and the physiological index data to obtain assessment test results. The invention further provides an assessment test device. According to the assessment test method, an assessment and/or comparation system of the timber architecture room environment is built, living environment, working environment and life quality of human beings are beneficially improved, and superiority of different kinds of timber architectures is beneficially and deeply developed.