The invention relates to the technical field of medicine production, in particular to a preparation method of an artificial bear gall powder preparation. The preparation method comprises the followingsteps of S1, preparing raw materials: poultry and livestock gall powder, beta cyclodextrin, cane sugar, dextrin, steviosin, menthol, peppermint oil, magnesium stearate, talcum powder, ethanol, starch, licorice root extractum powder, stevioside, PVA-4000, PVA-6000, glycerine, polyvinylpyrrolidone, hydroxypropyl methyl cellulose, hydroxyethyl cellulose, polyethylene glycol, dibutyl phthalate, a coloring agent and silicon dioxide; S2, dissolving the poultry and livestock gall powder with a buffered solution, performing centrifugation under the condition of 11000g, performing repeated filtrationto obtain filter residues and filtrate, and performing freeze drying and preservation on the filter residues; and S3, loading the filtrate in a reactor loaded with 7[alpha]-hydroxysteroid dehydrogenase. According to the preparation method disclosed by the invention, the artificial bear gall powder is designed to be a raw material medicine, and the artificial bear gall powder preparation is developed, so that the problem that natural bear gall raw materials are valuable and scarce is solved, and besides, a new medicine preparation is provided for human.