Method for preparing piper laetispicum extract, extract and its use
A technology for the preparation of betelwort extracts and extracts, which can solve the problems of large damage to active ingredients, high difficulty in experimental operations, and poor pharmacological activity of extracts, and achieve improved The content of amide alkaloids and the effect of improving the biological activity of the extract
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Embodiment 1
[0013] Example 1 , the preparation of betel leaf extract
[0014] As shown in Table 1, the roots, rhizomes, canes, leaves, fruits or whole plants of the betelwort were taken respectively to prepare the betelew extract, as follows:
[0015] Sample 1: Cut the roots and rhizomes of the betelwort into sections, add about 80% ethanol and soak at room temperature for about 24 hours, then add ethanol with the same concentration to 15 times the amount of medicinal materials, and percolate to obtain a crude extract. Slowly add about the same amount of warm water at 60°C and mix well, then put the diluted percolation liquid on the D101 macroporous adsorption resin column, and elute with 50% and 85% ethanol respectively after flowing through, and collect 85% ethanol to elute The liquid was concentrated and dried at ≤70°C to obtain a refined extract.
[0016] Sample 2: Cut the roots and rhizomes of Betelwort into segments, add about 45% ethanol and soak at room temperature for about 48 ...
Embodiment 2
[0029] Embodiment 2, the extract of betel is used for preparing medicine
[0030] The No. 1-12 extracts obtained in Example 1 are used as pharmaceutical raw materials, and prepared into capsules, tablets, granules, powders, dropping pills, micropills, injections, powder injections, and oral liquid preparations according to conventional production methods in the pharmaceutical field , sustained-release and controlled-release preparations, targeted preparations and other dosage forms. In view of the fact that these methods all belong to the routine operations in the prior art, only sample No. 1 obtained in Example 1 is used as an example to prepare capsules, granules and tablets.
[0031] 2-1. Capsules
[0032] prescription:
[0033] Extract 20g;
[0034] Corn starch 170g:
[0036] Preparation method:
[0037] The extract is mixed with starch and magnesium stearate, sieved, evenly filled into 1000 prefabricated empty capsules and sealed. Eac...
Embodiment 3
[0055] Embodiment 3, antidepressant effect-forced swimming test
[0056] Get the mice into random groups, and give the test drug as shown in Table 2 respectively, wherein the numbering of the drug corresponds to the sample number of embodiment 1 (the same below), and with normal saline as a control, the positive control group is venlafaxine. Give the test drug for 14 consecutive days, once a day, 60 minutes after the administration on the 14th day, put the animals in a glass cylinder with a diameter of about 10 cm, a height of about 20 cm, and a water depth of 8-10 cm, and the water temperature is 22-24 °C . Starting from the 2nd minute, record the accumulated time without moving for 4 minutes. The results are shown in Table 2.
[0057] Table 2
[0058] drug *
Dose (mg / kg / d)
n
Cumulative immobility time (s) X±SE
CK-
-
10
83.94±6.89
CK+
20
10
21.87±4.56 **
1
10
...
PUM
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