Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of total flavonoid extract with antidepressant effect and its preparation method and application

A technology of total flavonoids and extracts, applied in the field of medicine, can solve problems such as side effects, and achieve the effects of simple steps, saving raw materials, and significant antidepressant activity

Active Publication Date: 2022-03-15
宁夏超仁健康管理科技有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these drugs can play a certain antidepressant effect, they will produce a series of side effects along with their drug effects; and a survey shows that about one-third of antidepressant patients are forced to stop taking the drug because they cannot tolerate the side effects of the drug

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of total flavonoid extract with antidepressant effect and its preparation method and application
  • A kind of total flavonoid extract with antidepressant effect and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 A kind of preparation method of the total flavone extract with antidepressant effect, specifically comprises the following steps:

[0033] (1) According to the solid-to-liquid ratio of 1:8, add ethanol with a volume concentration of 95% to the dodder medicinal material, heat and reflux extraction for 3 times, each time for 2 hours, filter and combine the extracts, and concentrate under reduced pressure at 65°C until there is no alcohol smell;

[0034] (2) Get the concentrate in step (1) and carry out separation and purification on a polyamide chromatographic column, elute with ethanol solution with volume concentration of 30% and 70% respectively, and collect ethanol elution with volume concentration of 30% and 70% liquid, mixed and concentrated to obtain a concentrated solution.

[0035] (3) Add water to the concentrated solution obtained in step (2), stir and dilute it, then let it stand for 6-8h, and centrifuge (3000-5000rpm, 30min) to collect the precip...

Embodiment 2

[0037] Embodiment 2 A kind of preparation method of the total flavone extract with antidepressant effect, specifically comprises the following steps:

[0038] (1) According to the solid-to-liquid ratio of 1:8, add ethanol with a volume concentration of 95% to the dodder medicinal material, heat and reflux extraction for 3 times, each time for 2 hours, filter and combine the extracts, and concentrate under reduced pressure at 65°C until there is no alcohol smell;

[0039] (2) Get the concentrate in step (1) and carry out separation and purification on a polyamide chromatographic column, elute with ethanol solution with volume concentration of 40% and 80% respectively, and collect ethanol elution with volume concentration of 40% and 80% liquid, mixed and concentrated to obtain a concentrated solution.

[0040] (3) Add water to the concentrated solution obtained in step (2), stir and dilute it, then let it stand for 6-8h, and centrifuge (3000-5000rpm, 30min) to collect the precipit...

Embodiment 3

[0042] Embodiment 3 antidepressant experiment

[0043] 1. The modeling method of chronic stress-stimulated depressed mice in this example is:

[0044] Before the depression modeling, the experimental mice were given 2% sugar water for 3 days to adapt. After 3 days, the mice in the normal group were reared separately from the mice in the other groups. Food and water should be fasted during the sugar water preference experiment, and they were free to eat at other times. The model animals were subjected to chronic mild stimulation for 3 weeks, including swimming in cold water (15°C, 6min); tail clamping (1min); water deprivation (24h); fasting (24h); °); wet rearing (100ml water was added to the bottom of the cage with sawdust); overnight lighting stimulation. That is, the mice received one stimulus every day, each stimulus was guaranteed to be carried out randomly but not continuously, and the number of overnight lighting stimulations was at least twice a week.

[0045] 2. St...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a total flavonoid extract with antidepressant effect and a preparation method and application thereof. The total flavonoid extract is prepared according to the following preparation method: adding an extraction solvent to dodder seed powder, heating and refluxing for extraction, combining filtrates, and concentrating to obtain The extract; the extract is added to the polyamide column and the macroporous resin column respectively for chromatography, and the obtained eluate is concentrated to obtain the total flavonoid extract of Dodder. The total flavonoids extract prepared by the invention has high purity, and the experimental results show that the total flavonoids of Dodder can obviously shorten the immobility time of TST and FST of CUMS mice without affecting their autonomous activities, indicating that the total flavonoids of Dodder has strong anti-inflammatory properties. Depressive effect and no central excitatory or inhibitory effect. Moreover, total flavonoids from Dodder can activate the TXNIP / NLRP3 inflammasome signaling pathway, which proves that total flavonoids from Dodder can exert antidepressant effect by regulating the TXNIP / NLRP3 inflammasome pathway.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a total flavonoid extract with antidepressant effect and its preparation method and application. Background technique [0002] Depression is one of the most common mental illnesses. It is characterized by significant and persistent depression, often accompanied by serious psychological and physical disorders, and is extremely harmful to human health. The world invests a lot in its research and development every year, bringing great benefits to the society. serious burden on the family. In addition, patients with depression are often accompanied by changes in eating patterns related to insomnia, lethargy, overeating, weight loss or anorexia, and more serious symptoms include some extreme symptoms such as circadian rhythm and endocrine disorders. At present, there are at least 90 million depression patients in my country, and only 5% of them have received treatment; at the same t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/39A61K36/43A61P25/24A23L33/105
CPCA61K36/39A61K36/43A61P25/24A23L33/105A61K2236/333A61K2236/39A61K2236/55A61K2236/51A23V2002/00A23V2200/322A23V2250/2116
Inventor 王汉卿佟月唐蓉蓉陶伟伟刘颖赵建军雍婧姣王庆贺凯张霞冯军王建寰高晓娟付慧玲柳全霞曾亮杨彩艳
Owner 宁夏超仁健康管理科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com