Construction of geldanamycin gene engineering high yield strain

A technology of geldanamycin and high-yield strains, applied in the direction of microorganism-based methods, fungi, microorganisms, etc., can solve problems that have not been reported before, and achieve significant social and economic benefits
CN100393867CInactive Publication Date: 2008-06-11MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
Publication Date
2008-06-11
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention relates to geldanamycin high yield engineered bacteria strain construction, It is specificly described as follows: two groups of AHBA synthase gene cluster AHBA-shn gene discovered in water-absorbing streptomyces are destroyed by gene blocking technology due to likely participation in naphthoquinone type ansamycin compound biosynthesis. The present invention increases benzenquinone type GDM precursor (B-AHBA) supply and metabolic flow to promote GDM ferment yield by blocking or reduceing content of naphthoquinone type ansamycin compound precursor (N-AHBA). It proves that block mutant strain promote near 186% in yield to original strain.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field:

[0001] The invention relates to the application of genetic engineering technology in the construction of antibiotic high-yield bacterial strains. Background technique:

[0002] Geldanamycin (geldanamycin, GDM) producing bacterium-Streptomyces hygroscopicus 17997 (Streptomyces hygroscopicus 17997) is isolated from Chinese soil by the Institute of Pharmaceutical Biotechnology, Chinese Academy of Medical Sciences [Tao Peizhen and other Chinese Journal of Antibiotics, 1997, 22( 5): 368]. Geldanamycin has antitumor and broad-spectrum antiviral effects. Recent studies have shown that GDM can specifically inhibit the function of heat shock protein 90 (Hsp90). Hsp90 is a chaperone molecule for many signaling proteins. GDM can indirectly affect the function of intracellular signaling proteins by inhibiting Hsp90. Therefore, GDM is expected to become a potential anti-tumor and anti-viral drug. The novel mechanism of action of GDM determines the potential scientifi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More