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High purity pharmaceutical stevioside preparation method

A technique of steviol glycosides and column chromatography, which is applied in the field of purification, can solve the problems such as failure to achieve, and achieve the effect of high practical application value.

Inactive Publication Date: 2008-06-18
哈尔滨绿叶科技开发有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, JECFA believes that the purity of steviol glycosides used as sweeteners must be above 95%; the purity of steviosides currently produced in China is only 80%, which has not yet met the requirements of the pilot project

Method used

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  • High purity pharmaceutical stevioside preparation method
  • High purity pharmaceutical stevioside preparation method
  • High purity pharmaceutical stevioside preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0041] The present invention is described in more detail below:

[0042] 1. Material: stevioside coarse powder.

[0043] 2. Instruments and equipment:

[0044] BT124S one ten thousandth electronic balance;

[0045] Glass chromatography column (diameter 5.0cm x length 80.0cm);

[0046] glass test tube;

[0047] SBSZ100 CNC drop counting automatic partial collector;

[0048] UV-1600 ultraviolet-visible spectrophotometer.

[0049] 3. Reagents:

[0050] (1) Reagents for column chromatography: column chromatography for the first time: Al 2 o 3 , the first mobile phase (acetone:petroleum ether=1:1), the second mobile phase (100% ethanol); the second column chromatography: silica gel (100 μm), mobile phase (20% ethanol).

[0051] (2) Reagents for qualitative detection: steviol glycoside standard substance (300 times sucrose sweetness st and 450 times sucrose sweetness RA), glucose, maltose, n-butanol, acetic acid, ether, water, 50% sulfuric acid .

[0052] 4.Al2 o 3 Purifi...

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Abstract

The invention relates the preparing method of sweet chrysanthemum glycoside, comprising Al2O3 chromatography purification, compression and silica gel column chromatography purification. The first chromatography uses Al2O3 as fixed phase, removing amylaceum, barley sugar, protein and coloring matter, and collecting eluent. The second chromatography uses silica gel as fixed phase, removing impurity and collecting eluent to concentrate. The sweet chrysanthemum content is up to 95-98%. The invention has the advantages of high JECFA requirement and good application value.

Description

1. Technical field [0001] The present invention relates to a purification method, in particular to a purification method of steviol glycosides. 2. Background technology [0002] Purpose and significance: There are three main categories of sweeteners in industrial production: [0003] The first category is chemical synthesis products: saccharin, cyclamate, aspartame, etc.; [0004] The second category is chemically modified natural vegetable sugars, such as xylitol and mannitol; [0005] The third category is natural vegetable sugar. Natural plant sugars also include energy metabolism (sucrose, maltose, fructose, glucose, etc.), non-energy metabolism (licyrrhizin, steviol glycoside, etc.). [0006] With the continuous improvement of human living standards, people are more inclined to use natural products with higher safety: Glycyrrhizin and Steviol Glycoside, so Glycyrrhizin and Steviol Glycoside have broad application prospects in future life. [0007] Stevioside has the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/24C07H1/08
Inventor 郝再彬原益山苍晶杨丹王贵民一井真比古
Owner 哈尔滨绿叶科技开发有限责任公司