Biological oxygen demand microbe sensor sensitive membrane activating process and automatic activating apparatus

Abstract

The invention relates to a biochemical oxygen demand microbial sensor sensitive membrane activation method and an automatic activation device, relating to a microbial sensor, and provides a manual activation method, an automatic activation method and an automatic activation device for the BOD microbial sensor sensitive membrane. When using the manual method, use Soak the sensitive membrane in GGA solution until the fluorescence signal is stable, drain the GGA solution, and aerate the sensitive membrane until the fluorescence signal is constant; add new GGA solution to soak the sensitive membrane until the dynamic equilibrium is reached, and repeat the soaking-aeration step for multiple activations. The automatic activation device is provided with a water tank, a pump, a control circuit, an activation tank, a liquid level electrode and a solenoid valve. When using the automatic method, add GGA solution to the water tank, immerse the pump in the GGA solution; control the soaking and aeration time of the sensitive membrane, put the sensitive membrane into the activation tank card slot; then pump the GGA solution in the water tank into the activation tank In , the sensitive membrane was soaked in the GGA solution, and after aeration, the soaking and aeration steps were repeated.

Description

technical field [0001] The invention relates to a microbial sensor, in particular to an activation method and an automatic activation device which can be used for a biochemical oxygen demand (BOD) microbial sensor sensitive film. Background technique [0002] Biochemical oxygen demand (Biochemical Oxygen Demand, BOD) is one of the most commonly used and most important indicators in the process of water quality evaluation. The currently widely used 5-day biochemical oxygen demand standard dilution method is a standard method formulated by the American Public Health Association in 1936. However, the traditional measurement method not only requires time-consuming and complicated operation steps such as 5-day cultivation, but also the accuracy of the measurement has a lot to do with the proficiency and experience of the operator, which cannot realize real-time and online monitoring of environmental water samples. Therefore, research on BOD microbial sensors and related instrume...

AI Technical Summary

Problems solved by technology

It can be seen from the above that the activation of microbial pellicles is an indispensable step in the rapid assay of microbial sensors, and the preservation and activation methods of microbial pellicles similar to existing industry standards have the following disadvantages: (1) It takes a long time and requires 48h or even a few days

(2) After the biofilm is activated by PBS, it needs to continue to be installed on the surface of the electrode for activation. It can only be used after the signal is stable, which will affect the use of the sensing device and the continuous measurement of samples.

(3) If the bacterial film is used continuously for several hours, it needs to be replaced with a new film and reactivated, which will affect the continuity of the measurement and the reproducibility and stability of the measurement results

These tasks need to be done manually on the sensor, which is time-consuming, cumbersome, and occupies the sensing device, affecting the continuity and timeliness of detection

So far there is no automatic activation device for microbial sensing membranes specially used for biochemical oxygen demand (BOD) microbial sensors

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