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Use of intergrowth of mycorrhiza fungi for transfecting beneficial gene and plant rhizosphere

A technology of mycorrhizal fungi and arbuscular mycorrhizal fungi, applied in the direction of climate change adaptation, introduction of foreign genetic material using vectors, and other methods of inserting foreign genetic material, which can solve the problem that the ability of biocontrol cannot fundamentally prevent and control plant diseases and other issues, to achieve the effect of strong feasibility and wide application fields

Inactive Publication Date: 2007-08-01
INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

However, the biocontrol ability of arbuscular mycorrhizal fungi cannot fundamentally achieve the effect of preventing and controlling plant diseases.

Method used

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  • Use of intergrowth of mycorrhiza fungi for transfecting beneficial gene and plant rhizosphere
  • Use of intergrowth of mycorrhiza fungi for transfecting beneficial gene and plant rhizosphere
  • Use of intergrowth of mycorrhiza fungi for transfecting beneficial gene and plant rhizosphere

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Experimental program
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Embodiment Construction

[0030] Process route of the present invention is as follows:

[0031] (1) Establishment of the in vitro symbiotic system of arbuscular mycorrhizal fungi (see Figure 2)

[0032] (2) Construction of the plasmid containing the resistance gene (see Figure 3)

[0033] (3) Genetic transformation of arbuscular mycorrhizal fungi (see Figure 4)

[0034] (4) Infect plants with arbuscular mycorrhizal fungus spores successfully transfected with beneficial genes (disease resistance, insect resistance, efficient utilization of nutrients, etc.) to form a symbiotic system, which plays a role in resisting plant rhizosphere pests and diseases and improving quality.

[0035] Specific steps:

[0036] 1. Establishment of in vitro symbiotic system of arbuscular mycorrhizal fungi

[0037]Arbuscular mycorrhizal fungi are a type of obligate symbiotic fungi that rely on live nutrients. They must form a symbiotic relationship with plant roots to complete the entire life cycle. Currently, they cannot ...

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Abstract

The invention discloses an application of commensalism of transfected mycorrhiza fungi of significant gene and plant rhizosphere. It comprises: transfecting gene good for plant disease resistant, insect resistant and nutrient high- efficiency avalability to bush mycorrhiza fungi to transfe plasmid in said gene onto bush mycorrhiza fungi spore, the bush mycorrhiza fungi will infect the plant root and form commensalism system, and the plant root will obtain resistance to pathogene and growth will be accelerated through significant gene expression. The invention can detect expression effect of bush mycorrhiza fungi under sterile and controllable condition through external triple culturing system (root system of plant, bush mycorrhiza fungi and detection for plant growth index and pathogene in root system of plant).

Description

technical field [0001] The invention belongs to the application of safe transgenic technology in plants, and specifically utilizes arbuscular mycorrhizal fungus engineering bacteria that transfer beneficial genes such as disease resistance, insect resistance, and high-efficiency nutrition utilization to coexist with plant rhizosphere to realize resistance to plant rhizosphere pests and diseases , Improve the efficient use of soil water and nutrients by the root system. Background technique [0002] The plant rhizosphere is a very complex soil environment. Plants are often attacked by rhizosphere organisms including bacteria, fungi, nematodes, and larvae of some insects during the growth process, resulting in crop yield loss, product quality deterioration, and plant diseases. Just taking plant parasitic nematodes as an example, they cause about 12% loss to food and fiber plants and more than 20% loss to fruit trees and vegetables every year, and the annual loss of agricultur...

Claims

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Application Information

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IPC IPC(8): C12N15/09C12N15/82C12N15/87
CPCY02A40/146
Inventor 肖翔吴李君吴跃进余增亮王军
Owner INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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