Portable bilayer lipid membrane electrochemical gene sensor

An electrochemical, portable technology for bioelectrochemical sensors

Inactive Publication Date: 2007-08-01
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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Problems solved by technology

[0005] In order to solve the technical problem in the prior art that often needs to be marked in the process of detecting DNA, the present invention provides a small-volume portable double-layer lipid membrane electrochemical gene sensor. By modifying the electrode surface, the DNA fragments or probes are immobilized on the surface of the electrode

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  • Portable bilayer lipid membrane electrochemical gene sensor
  • Portable bilayer lipid membrane electrochemical gene sensor

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[0019] The present invention will be further described in detail below in conjunction with the drawings and specific embodiments.

[0020] As shown in Figure 1, Figure 2 and Figure 3, the portable double-layer lipid membrane electrochemical sensor of the present invention is composed of a miniature electrochemical detector and a detection electrode 3. The miniature electrochemical detector includes a potentiostatic module 1 and a central Control module 2; The constant potential module 1 is composed of an error amplifier with a high input impedance operational amplifier as the core. The reference electrode potential is compared with a given potential and sent to the bandpass filter and power frequency limiter through the error amplifier Process, remove noise and interference, and output to the detection electrode through the power amplifier; the central control module 2 includes a data acquisition unit 21, a range switching unit 22, an input display unit 23 and a single-chip microc...

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Abstract

This invention discloses one portable double resin film electro-chemical sensor, which comprises level constant module, central control module and test electrode, wherein, the constant level module is composed of high input resistance computation amplifier as center error amplification device; reference electrode and given electrode are comparing to be sent to band filter and work frequency limit wave device for process to remove noise and interference through power amplifier to output test electrode; central control module also comprises data collection unit, progress switch unit, input display unit and single machine.

Description

technical field [0001] The invention relates to a bioelectrochemical sensor, in particular to a portable electrochemical gene sensor. Background technique [0002] At present, DNA detection usually adopts PCR detection method, which has high sensitivity, but also has its own shortcomings. Because PCR detection method enlarges a very small amount of DNA by a million times, false positives are more common while high sensitivity . [0003] Since the 1990s, various biological detection systems based on biosensor technology have emerged rapidly, and some fast and modern technology detection methods have emerged continuously. This technology has also been gradually applied to the detection field. The electrochemical detection method has the advantages of simple sample preparation, The test time is short, the cost is low, and the sensitivity is high, and the DNA does not need to be labeled. Electrochemical gene sensors are the product of the combination of biotechnology and elect...

Claims

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Application Information

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IPC IPC(8): G01N27/403C12Q1/68
Inventor 周焕英王军波高志贤崔大付刘楠
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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