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Bacillus alcaligenes strain for degrading ortho-nitrophenol and preparing method

A kind of technology of o-nitrophenol and Alcaligenes, applied in the field of microorganisms

Inactive Publication Date: 2007-08-15
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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Problems solved by technology

At present, there is no report on the degradation of o-nitro compounds by bacteria of the genus Alcaligenes Gram-negative at home and abroad

Method used

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  • Bacillus alcaligenes strain for degrading ortho-nitrophenol and preparing method

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Embodiment Construction

[0029] Preparation, isolation and cultivation of Alcaligenes:

[0030] a. Preparation of inorganic salt medium

[0031] The composition is disodium hydrogen phosphate (Na 2 HPO 4 12H 2 0) 14.3g; Potassium dihydrogen phosphate (KH 2 PO 4 ) 3.0g; manganese sulfate (MnSO 4 ·H 2 O) 0.28mg; ferrous sulfate (FeSO 4 ·7H 2 O) 0.3mg; Magnesium sulfate (MgSO4) 0.06mg; Calcium chloride (CaCl 2 ) 1mg; copper sulfate (CuSO 4 ) 0.05mg and zinc sulfate (ZnSO 4 ) 0.05 mg. Make it up to 1000ml with double distilled water, adjust the pH to 7.0, and autoclave at 121°C for 30 minutes.

[0032] b. Enrichment of strains

[0033] Take a sample from the activated sludge of a chemical plant, mix the soil sample into the above-mentioned inorganic salt medium to prepare a 20% suspension, and then inoculate the suspension with a 5-10% volume inoculum into fresh inorganic salt containing o-nitrophenol In the salt medium, the temperature is kept at 28-30° C., and the shaker is cultivated for ...

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Abstract

The invention discloses a preparing method of bacillus alcaligenes strain Alcaligenes sp., CCTCC No.M206121 of degraded o-nitrophenol, which comprises the following steps: allocating inorganic salt culture medium with dibasic sodium phosphate, monobasic potassium phosphate, manganese sulfate, copperas, addex-magnesium, calcii chloridum, blue copperas, white copperas and hydrogen dioxide solution; adjusting pH value; sterilizing under high temperature; enriching bacterial; sampling from sludge; allocating suspending liquid with soil sample and inorganic salt; seeding into ortho-nitrophenol fresh organic salt culture medium with finite volume seeding quantity; keeping the temperature; culturing on shaker; getting enriched culture; separating and purifying enriched culture in LB solid culture medium. This invention can makes the degraded reach to 99%.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to an Alcaligenes sp. NyZ215 degrading o-nitrophenol, and to a preparation method of the Alcaligenes sp. Alcaligenes NyZ215 can use o-nitrophenol as the only carbon source, nitrogen source and energy source for growth, so it can completely degrade the pollutant o-nitrophenol. Background technique [0002] At present, nitroaromatic compounds are produced in large quantities and are widely used in industries such as industry, agriculture, and pharmaceutical industry, and are closely related to people's lives. Most of them are poisonous and harmful compounds to humans, and they have accumulation and amplification effects in the food chain, and are teratogenic, mutagenic, and cancer-inducing, threatening human health and survival. Artificially synthesized nitroaromatic compounds will inevitably leak into the environment when they are used, and they will leak in lar...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/05
Inventor 周宁一肖毅刘虹王淑君
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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