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Rubber tree embryonic connective long-term subproliferation method

A technology of embryogenic tissue and sub-generation proliferation, applied to plant cells and other directions, can solve the problems of low plant regeneration frequency, difficult long-term sub-generation preservation, and high production cost, so as to improve experimental and production efficiency, shorten embryonic time, and improve The effect of embryogenesis efficiency

Inactive Publication Date: 2007-08-29
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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AI Technical Summary

Problems solved by technology

At present, the main problems in the cultivation of anthers and inner integuments are: the explants are limited by the flowering and fruiting time of rubber, the embryo formation rate of dedifferentiated callus is low, and it is difficult to preserve for a long time, so the plant regeneration frequency is low. The high production cost cannot meet the large demand for tissue culture seedlings in production and the needs of biotechnology experiments such as establishing an efficient genetic transformation system

Method used

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Examples

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Embodiment Construction

[0017] The present invention will be described in detail below in conjunction with examples.

[0018] The long-term subculture and multiplication method of rubber tree embryogenic tissue designed by the present invention includes the selection and processing process of subculture embryogenic tissue, the subculture proliferation process of embryogenic tissue, the "refolding" process of embryogenic tissue, and the embryonic tissue after subculture. The process by which sexual tissues are induced into mature embryos.

[0019] 1. Selection and processing of subculture embryogenic tissue On the basis of rubber tree anther tissue culture or inner integument tissue culture, take anther or inner integument dedifferentiated callus after embryo induction for about 3 to 4 months and then primary Fresh and healthy embryogenic tissues were subcultured. Experiments show that embryogenic tissue can be directly subcultured on the subculture medium at the heart-shaped embryo stage and before....

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Abstract

The invention discloses a gum tree embryonic tissue long term fertilizing method that includes selecting and processing heir embryonic tissue, fertilizing process of heir embryonic tissue, restoring process of heir embryonic, and inducing maturing process of embryonic tissue after inheriting. The invention has the advantages of simple technology flow, low producing cost, and high fertilizing of embryonic tissue, and long time of inheriting. It has crucial application values.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for the rubber tree embryonic tissue to achieve proliferation and long-term subculture preservation through plant tissue culture technology. Background technique [0002] Brazilian rubber tree (Hevea brasiliensis) belongs to Hevea genus Euphorbiaceae. It is an important economic crop widely planted in hot areas. Because of its high latex yield, good quality and easy access, it has commercial development value and has become the main source of natural rubber in the world. Since the rubber tree is a perennial tree, the conventional breeding cycle is long, and the selective breeding is greatly restricted. In order to broaden the breeding methods of Hevea brasiliensis and improve its quality, since the 1950s, rubber breeders have begun to study its tissue culture technology, especially since the 1970s, my country, Malaysia, France, India, Indonesia, Sri Lanka and other countr...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 彭明赵辉崔百明赵平娟
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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