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Cocktail vaccine for anti immune tolerance and immunodeficiency virus and its application

A technology of immune tolerance, cocktail, applied in the field of immunology

Inactive Publication Date: 2012-10-10
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] According to the above research results and the fact that vaccines for immune tolerance and immunodeficiency viruses have not yet made breakthroughs at home and abroad, and are facing severe social needs and technical challenges, we promptly propose a new vaccine development strategy, namely: cocktail A new strategy for the development of anti-immune tolerance and immunodeficiency viruses, the main point of which is to combine multiple anti-viral elements in a vaccine according to the characteristics of virus infection and the response characteristics of the body

Method used

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  • Cocktail vaccine for anti immune tolerance and immunodeficiency virus and its application
  • Cocktail vaccine for anti immune tolerance and immunodeficiency virus and its application
  • Cocktail vaccine for anti immune tolerance and immunodeficiency virus and its application

Examples

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Embodiment 1

[0073] Embodiment one: the therapeutic cocktail vaccine of HBV

[0074] According to the characteristics of immune tolerance caused by HBV infection, its therapeutic cocktail vaccine is a single-vector vaccine or multi-vector combination vaccine containing multiple components. Cocktail vaccines have combined advantages over the therapeutic goals achieved by individual components. This example provides a construction and combination method of a multi-vector cocktail vaccine and a construction method of a single-vector cocktail vaccine, but the content contained in this patent is not limited to the combination method of the cocktail vaccine disclosed in this example.

[0075] 1. Multi-vector HBV therapeutic cocktail vaccine ( figure 1 )

[0076] Composition and Application of Multi-vector HBV Therapeutic Cocktail Vaccine

[0077] Vector 1: Recombinant adenovirus containing RNA interference sequence

[0078] Sustained virus replication is an important feature of immune tol...

Embodiment 2

[0130] Embodiment two: the therapeutic cocktail vaccine of HCV ( figure 1 )

[0131] 1. The multi-vector therapeutic cocktail vaccine of HCV

[0132] The construction strategy and method of the multi-vector therapeutic vaccine of HCV are similar to the multi-vector therapeutic cocktail vaccine of HBV, such as figure 1 As shown, the vector 1 is a recombinant adenovirus or an adenovirus library containing RNA interference elements. ABC in vectors 2 and 3 refers to IFN, the structural antigen capsid protein (core) of HCV, E1, E2, epitope-related SCT and multi-epitope fusion genes, gp96 coding sequence (SEQ ID NO: 3), Gp96N - Optional combination of core / E1 / E2 fusion genes.

[0133] In this example, the strategy for constructing recombinants related to some of the vectors is briefly described.

[0134] The effective sequence for inhibiting HCV expression was obtained using the same method as in Example 1 vector 1: 5'-CCCGCTCAATGCCTGGAG-3' (SEQ ID NO: 68), which was converted...

Embodiment 3

[0140] Embodiment three: HIV-1 prophylactic cocktail vaccine

[0141] The HIV preventive cocktail vaccine disclosed in the present invention is a DNA, adenovirus or vaccinia virus vaccine constructed from a combination of the following elements: (1) the heptapeptide repeat sequence HR212 that can block the fusion of the virus and the cell; (2) ) adjuvant molecules of gp96 or its amino-terminal fragment; (3) structural antigens env, gag and pol of HIV or their fusion genes fused with adjuvant and / or antigenic epitopes, multi-epitope fusion genes and antigenic epitopes of HIV Bit-MHC-I constructed SCT gene. Similar to Example 1 and Example 2, the preventive cocktail vaccine of HIV-1 disclosed in this patent comprises a multi-carrier cocktail vaccine ( figure 1 ) and single vector cocktail vaccine ( Figure 7 ). multivector cocktail vaccine figure 1 The combination of vector 2 and vector 3 related to the inhibition of HIV invasion, wherein ABC refers to the optional combina...

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Abstract

The invention provides a cocktail vaccine that is anti-immune tolerance and anti-immunodeficiency virus and its application. Said cocktail vaccine at least comprises two or more elements and any viral antigen recombinant protein and medical shaping. Said element is recombinant DNA and / or recombinant virus; it is chosen from (A) neutralizing antibody, receptor antagonist, membrane fusion blocking agent, cell factor coding sequence, and / or DNA interference sequence; (B) immunoadjuvants coded sequence; (C) antigen gene, viral antigen and immunoadjuvants fusion gene, viral antigen epitope coded sequence, multi-epitope fusion gene and / or viral antigen epitope -MHC-I SCT gene; (D) two seven-peptide multiplexed sequence HR1 and HR2 of membrana capsular is virus fusion protein and / or coded sequence of its polymer HR212. The expression carrier of all antiviral elements comprises recombinant DNA carrier, vaccinia virus carrier and / or adenoviral carrier.

Description

technical field [0001] The invention belongs to the field of immunology and vaccine development technology, in particular to a cocktail vaccine against immune tolerance and immunodeficiency virus and its application. Background technique [0002] 1. Immune tolerance virus and its immunological basis: Immunotolerant viruses are viruses that cause the body's immune system to become less responsive. Although the pathogenesis of each virus is different, they all lead to diseases characterized by the persistence of the virus, the decline or disappearance of the ability of B cells and T cells to produce antibodies and cellular immunity. Among the many viruses that cause hepatitis, hepatitis B virus (HBV) and hepatitis C virus (HCV) are the two most important immune tolerance viruses. [0003] The immune tolerance virus can effectively escape the body's immunity and cause immune tolerance, so that the immune mechanism of the human body no longer produces an immune response to the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K39/295A61K39/395A61P31/18C12N15/861C12N15/863
Inventor 田波张玉霞刘长梅高福
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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