BIV indication cell line using enhanced green fluorescence protein as reporting gene

A technology of green fluorescent protein and indicator cell line, which is applied in the field of detection of cell lines infected by bovine immunodeficiency virus, can solve problems such as high subjectivity, and achieve the effects of simple and easy operation, reducing safety risks and easy operation.
CN101100675BInactive Publication Date: 2011-01-19NANKAI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANKAI UNIV
Publication Date
2011-01-19
Estimated Expiration
Not applicable · inactive patent
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Abstract

A Bovine Immunodeficiency Virus BIV infected cell system and use are disclosed. The process is carried out by expressing reinforced EGFP under BIV infectious specific condition, constructing respondent Bovine Immunodeficiency Virus BIV infected reporting plasmid with nucleotide acid sequence in sequence table, transfecting it to BHK-21 cell, screening expressed cell system, mono-cloning and selecting high-expression EGFP negative cloning. It can be used to indicate BIV infection and activate BIV trans factor.
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Description

【Technical field】

[0001] The invention relates to a cell line for detecting bovine immunodeficiency virus infection, a method for establishing the cell line and its specific application. 【Background technique】

[0002] Bovine immunodeficiency virus (BIV) and human immunodeficiency virus (HIV) belong to the genus Lentivirus of the Retroviridae family, and are highly homologous to the HIV genome. The relationship, pathogenic mechanism, etc. are very similar to HIV. It is used as an AIDS research model to explore the regulation of lentiviral gene expression, the interaction between lentivirus and other viruses after superinfection, and between viruses and host cells. It has high security. The establishment of BIV indicator cell lines is a powerful tool for studying the molecular biology of BIV.

[0003] The cell line that can be stably transfected by the virus with the indicator plasmid is called the indicator cell line. The genome of this cell line contains a reporter gene ...

Claims

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