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Method for checking mononucleotide polymorphism of CYP2C19 gene number six extron

A technology of CYP2C19 and single nucleotide polymorphism, applied in sugar derivatives, organic chemistry, DNA/RNA fragments, etc., can solve the problems of undiscovered reports and achieve great theoretical significance

Inactive Publication Date: 2007-10-31
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] Searching the existing literature, no report related to the sixth exon (C905G) SNP (single nucleotide polymorphism) of the CYP2C19 gene of the present invention was found

Method used

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Examples

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Embodiment Construction

[0015] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental method that does not indicate specific condition in the following examples, usually according to routine conditions, such as people such as Sambrook, molecular cloning: the condition described in the laboratory handbook (New York: Cold Spring Harbor Laboratory Press, 1989), or according to manufacture conditions recommended by the manufacturer.

[0016] step 1

[0017] Isolation and sequencing of a nucleic acid

[0018] Primer design:

[0019] Using Primer 5.0 software, a pair of allele-specific nucleic acid primers were designed using the GenBank database CYP2C19 gene (AY796203) No. 6 exon and exon-intron junction sequence as templates, and were synthesized by Invitrogen.

[0020] Primer information:

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PUM

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Abstract

The invention discloses a checking method of CYP2C19 gene sixth extron mononucleotide polymorphism and nucleic acid primer of a separating nucleic acid and an allele specificity, which is characterized by the following: assuring 905 site nucleic acid in SEQ ID NO: 1 sequence of human CYP2C19 gene sixth extron; checking mononucleotide polymorphism; possessing a separating nucleic acid, sequence in SEQ ID NO: 1 and 905 site as G; possessing a nucleic acid primer of allele gene specificity with length at 15-50bp; proceeding special cross; augmenting; getting 905 site mononucleotide polymorphism in SEQ ID NO: 1 with human CYP2C19 gene sixth extron.

Description

technical field [0001] The present invention relates to nucleic acid and its primers and detection method in the field of gene technology, specifically to an isolated nucleic acid, an allele-specific nucleic acid primer, and a single nucleotide polynucleotide (SNP) of the sixth exon of CYP2C19 gene. state-of-the-art detection method. Background technique [0002] Cytochrome oxidase P4502 C19 (CYP2C19), also known as S-mephenytoin hydroxylase, is a member of cytochrome P450. In the CYP2C family, six genes have been identified by cDNA cloning methods, namely CYP2C8 and CYP2C9 , CYP2C10, CYP2C17, CYP2C18, and CYP2C19, among which CYP2C19 catalyzes the oxidative metabolic reactions of various drugs commonly used in clinical practice, including proton pump inhibitors (PPI) such as omeprazole, and is the human liver cytochrome oxidase p450 enzyme A liver drug enzyme in the system. The genetic polymorphism of the enzyme activity affects the metabolism of many clinical drugs, and ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C07H21/00
Inventor 秦胜营贺林陈玲玲邢清和
Owner SHANGHAI JIAO TONG UNIV
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