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Use of bupleurum root total polyoses in the preparing of medicine for preventing and treating systematic lupus erythematosus

A technique for lupus erythematosus and total polysaccharide, which is applied in the preparation of medicine for preventing and treating systemic lupus erythematosus, total polysaccharide of Bupleurum chinensis, and the new medicinal use field of total polysaccharide of traditional Chinese medicine Bupleurum chinen The efficacy of lupus and its drugs and other issues

Active Publication Date: 2008-01-02
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Taking a comprehensive view of domestic and foreign research, there is no report on the efficacy of Bupleurum polysaccharides in preventing and treating systemic lupus erythematosus and its medicinal properties.

Method used

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  • Use of bupleurum root total polyoses in the preparing of medicine for preventing and treating systematic lupus erythematosus
  • Use of bupleurum root total polyoses in the preparing of medicine for preventing and treating systematic lupus erythematosus
  • Use of bupleurum root total polyoses in the preparing of medicine for preventing and treating systematic lupus erythematosus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take 100g of Bupleurum herb, extract by cold soaking with 95% ethanol, put the dregs in a ventilated place at room temperature to dry, then extract with hot water for 3 times, filter, combine the extracts, concentrate, centrifuge, and the supernatant is washed with S3 Chloroacetic acid removes free protein, centrifuges, and dialyzes the supernatant with water for 3 days, concentrates the dialysate to a small volume, adds ethanol to an alcohol content of 80%, centrifuges, precipitates and freeze-dries to obtain the total polysaccharide, and the product yield is more than 3%. The content exceeds 65%.

[0036] The Bupleurum medicinal material can be selected from Bupleurum chinense DC, Bupleurum scorzonerifolium Willd or Bupleurum smithii Wolff.

[0037] Table 1 shows the yield and content of total polysaccharides in Bupleurum chinensis.

[0038] Table 1

[0039] batch number

Embodiment 2

[0041] Thirty Kunming mice (16-20 g) were randomly divided into 5 groups (groups A, B, C, D, and E). Group A is the normal control group, group B is the model control group, group C is the SLE model group, group D is the Bupleurum polysaccharide group, and group E is the prednisone acetate group. For the initial immunization, group A was the normal control group, with saline injected subcutaneously at the base of the tail, group B was the model control group, and Freund's complete adjuvant was injected subcutaneously at the base of the tail, and the rest of the groups were subcutaneously injected with Campylobacter jejuni and complete Freund's adjuvant at the base of the tail. Injection, immunization induces SLE-like syndrome, and the injection volume of each mouse is 50 μl. On the 14th day after the initial immunization, booster immunization was given once: groups A and B were subcutaneously injected with normal saline, and the rest of the groups were subcutaneously injected ...

Embodiment 3

[0053] Forty-eight BALB / c mice (16-20 g) were randomly divided into 5 groups (groups A, B, C, D, E, and F). Group A is the normal control group (n=8), group B is the model control group (n=8), group C is the SLE model group (n=10), group D is the Bupleurum polysaccharide 30mg / kg group (n=8 ), group E is Bupleurum polysaccharide 15mg / kg group (n=8), group F is prednisone acetate group (n=8). For the initial immunization, group A was the normal control group, with subcutaneous injection of normal saline on the soles of the feet, group B was the model control group, and the soles of the feet were injected with Freund's complete adjuvant subcutaneously, and the rest of the groups were treated with Campylobacter jejuni combined with complete Freund's adjuvant. Plantar subcutaneous injection, immune induction of SLE-like syndrome, the injection volume of each mouse is 50μl. On the 14th day after the initial immunization, a booster immunization was given once: groups A and B were in...

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Abstract

The invention belong to Chinese traditional medicine field, relates to the application of bupleurum root polysaccharide for preparing medicine preventing systematic Cazenave's lupus. The invention separates polysaccharide extract from bupleurum root, the average productivity is 3.32%, and the polysaccharide content is more than 65%. It is showed by whole animal model test that the protective effect for hyper reaction and kidney injury for mice by said polysaccharide is distinctive, and it is proven that polysaccharide can prevent Cazenave's lupus syndrome. The bupleurum root polysaccharide can also be used to produce medicine preventing and treating self-immune disease.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a new medical application of total polysaccharide of Bupleurum bupleuri. It specifically relates to the use of Bupleurum total polysaccharides in the preparation of medicines for preventing and treating systemic lupus erythematosus. Background technique: [0002] Systemic lupus erythematosus (SLE) is an inflammatory connective tissue disease with unclear etiology. It mainly affects women and can also affect children. A multi-country and multi-center study showed that the current incidence of systemic lupus erythematosus is 12.5 / 100,000 to 39 / 100,000. SLE is generally regarded as a representative autoimmune disease, and it is a severe autoimmune disease that is refractory, recurrent, and has a high fatality rate. The phenotype of autoimmune abnormalities in SLE is highly diverse, almost involving the entire immune system, including immune tolerance defect, lymphocyte ap...

Claims

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Application Information

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IPC IPC(8): A61K36/233A61K31/715A61P37/02A61P37/08A61P17/00A61K125/00
Inventor 陈道峰力弘王铮徐晗章蕴毅
Owner FUDAN UNIV
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