Method for improving the seedling-becoming ratio hybridized with planting rice and different genome type wild rice

Abstract

The invention relates to a method, belonging to the plant cross breeding technology, which aims to solve the problems that the hybrid of improved cultivated rice and the wild rice with various genome types are difficult to germinate, and the culture seedling rate is low; the invention can greatly improve the culture seedling rate of wild planted distant hybrids immature embryo. The invention adopts the technical proposal that at the best culture period after the pollination hybridization of the female ben of the cultivated rice and the male ben of the wild rice with various genome types, fake hybrid grains are judged and removed, and real hybrid grains are selected, deprived of glumes, disinfected and directly put into a culture medium to be cultivated. The invention has the advantages that the purity of the cultivated wild planted hybrid seedlings is improved; meanwhile the immature embryo emergence rate can be stably improved from ordinary 5% to 50% to 35% to 70%; furthermore, the method also has the advantages of reduced cost, low technical difficulty and easy operation; thus the invention provides a standardized new method for the innovation of the germplasm resources of crop genetic breeding.

Description

Technical field: [0001] The patent of the invention belongs to the field of innovation of rice germplasm resources by utilizing wild rice with different genome types, and specifically relates to a method for improving the seedling rate of interspecies distant hybrid hybridization between cultivated rice and wild rice with different genome types. Background technique: [0002] Different genotypes of wild rice cover nine genomes of B, C, D, E, F, G, H, J, and K, which are widely distributed all over the world and have rich genetic diversity and many excellent traits. It is a natural rice gene The treasure house can provide abundant germplasm resources for broadening the genetic base of rice. For example, heavy glume wild rice (CCDD type, tetraploid): resistant to rice blast, resistant to pests such as Philippine water fly, stem borer and brown planthopper, high cold resistance, shade tolerance, good growth under low light conditions, and has great use in rice breeding value. ...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the problem of long genetic relationship between cultivated rice and wild rice with different genotypes and the difficulty in breeding with large differences in genetic characteristics. method, it can distinguish true and false hybrids, quickly screen out the cultivated rice parents with high seedling rate, and can accurately determine the best time to take out and cultivate the true hybrids, so as to achieve the purpose of improving the seedling rate of young embryos