General-purpose highly effective eukaryon expression carrier p3I-GFPN and mastitis-resisting transgene carrier constructed by the same

Abstract

The invention discloses a universal efficient eukaryotic expression vector p3I-GFPN and an anti-mastitis transgenic vector which is constructed by employing the vector. The anti-mastitis transgenic vector pCMV / CSN2-hLY-hIFA constructed by the invention can express green fluorescent protein at a high level in mammary gland cell. In the mammary gland cell of lactation period, initiation effect exerted by promoter of CSN2 enables the mammary gland cell of transformed pCMV / CSN2-hLY-hIFA to secrete a plurality of human lysozyme and human interferon A, and achieve target protein via breast milk separation and preferably fight against mastitis of livestock simultaneously.

Description

technical field [0001] The invention belongs to the field of genetic engineering and relates to the construction of a eukaryotic expression vector, in particular to a high-efficiency eukaryotic expression vector p3I-GFPN and a high-efficiency anti-mastitis transgene vector constructed by the vector. Background technique [0002] The construction of high-efficiency eukaryotic expression vectors is the research focus and hot spot in animal transgenic technology at present. So far, the expression vectors used in animal genetic engineering can be roughly divided into plasmid vectors, viral vectors and artificial chromosomes. A good expression vector for animal genetic engineering must have the ability to efficiently integrate into the animal genome and express foreign genes in animals. Although viral vectors have solved the problem of efficient integration, the problem of efficient expression of foreign genes remains to be seen. solve. The intervening sequence (IVS) is a DNA se...

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Problems solved by technology

At present, the treatment of mastitis mainly involves the use of antibiotics and vaccines, and the milk of animals after using antibiotics cannot be marketed because it contains a large amount of antibiotics. What is more serious is that the milk production of many animals suffering from mastitis is severely reduced or even unable to produce milk for life; vaccines Although it can treat mastitis better, the vaccine is prone to failure in a short period of time due to the high variability of bacteria

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