Selective enrichment and separation method for dyestuff lignin in kudzu root crude extract

A technology of genistein and separation method, which is applied in the field of selective enrichment and separation of genistein in the crude extract of kudzu root, can solve the problems of solid phase extraction that have not been reported, and achieve the effect of broad application prospects

Inactive Publication Date: 2008-07-02
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

In recent years, some scholars have used molecular imprinting technology for the enrichment and separation of active ingredients of traditional Chinese medicine and obtained sa...

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  • Selective enrichment and separation method for dyestuff lignin in kudzu root crude extract

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Embodiment 1

[0017] Pack 200 mg of genistein molecularly imprinted polymer particles with a particle size of 38-65 μm in a solid-phase extraction column with an inner diameter of 1 cm and a length of 10 cm, and plug the bottom and upper parts of the column with glass frits. The extraction experiments were carried out on a self-made solid phase extraction device. Before loading, the extraction column was activated with 5ml of methanol and 5ml of acetonitrile in turn; then 1ml of 0.378mg / ml kudzu root total extract in acetonitrile solution was loaded, and then rinsed with 2ml of 50% water in acetonitrile solution and 1ml of 5% water in acetonitrile solution , and finally eluted with 2ml of 0.2% TEA in methanol solution, the eluent was blown dry with nitrogen gas, and then 1ml of 60% water in acetonitrile solution was used to make up the mobile phase, and then the genistein was subjected to chromatographic analysis.

[0018] Comparison of chromatograms before and after molecularly imprinted s...

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Abstract

The invention relates to a concentration and separation method of microcomponent in a complex system, in particular to the selective concentration and separation method of genistein in radix puerariae crude extract. The steps adopted are as follows: a) genistein molecularly imprinted polymer microspheres are employed as filler to prepare solid phase extraction column; b) the solid phase extraction column prepared in the step a is activated and sampled and then the solid phase extraction column is leached by using acetonitrile solution with water accounting for 49-51 percent of volume ratio and acetonitrile solution with water accounting for 4.9-5.1 percent of volume ratio in sequence, and then the leached solid phase extraction column is eluted by methanol solution with water accounting for 0.18-0.22 percent of volume ratio, thus obtaining finished products through drying the eluent. The invention has the advantages that: after the radix puerariae ethanol crude extract is extracted through molecularly imprinted solid phase, a clean chromatogram is obtained after removing most disturbance factor and the recovery ratio of the genistein reaches above 70 percent. Meanwhile, glycitein is reserved obviously. The invention has wide application prospect on the pretreatment of radix puerariae crude extract samples.

Description

technical field [0001] The invention is an enrichment and separation method for trace components in a complex system, specifically a molecular imprinted solid phase extraction technology with high selectivity and high affinity for genistein. Background technique [0002] Isoflavones are a class of natural compounds present in legumes. Many studies have shown that isoflavones have anti-cancer, anti-oxidation, scavenging free radicals, lowering cholesterol and estrogen activity (J.Agric.Food Chem.41(1993 ) 1961; Chem. Pharm. Bull. 36 (1988) 2090; Lipids, 14 (1979) 535; J. Nutr. 84 (1964) 201; Proc. Natl. Acad. Sci. U.S.A., 90 (1993) 2690). As phytoestrogens, isoflavones can not only alleviate menopausal symptoms caused by estrogen deficiency, but also have no toxic side effects (Food Res.Int.26(1993) 131; Arch.Intern.Med.16(2001) 1161), Therefore, it has been paid more and more attention by people. [0003] Genistein is an isoflavone compound with high estrogenic activity, w...

Claims

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Application Information

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IPC IPC(8): C07D311/36B01J20/285A61K36/48A61K125/00
Inventor 梁鑫淼王金成徐青薛兴亚章飞芳张岩
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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