Kit and method for quantitative determination of ochratoxin A content in food product

A technology for qualitative detection of ochratoxin, applied in the field of immunoassay, can solve the problems of rare reports of small molecule compounds and blank detection of small molecule biotoxins, etc., and achieves the effects of wide application range, simple operation and high detection efficiency.

Inactive Publication Date: 2008-07-30
TIANJIN UNIV OF SCI & TECH
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  • Abstract
  • Description
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Problems solved by technology

At present, there are more than 20 kinds of commercial colloidal gold kits for sale at home and abroad, such as the most common early pregnancy test strips, gonorrhea test kits, etc. The application is rarely reported, and the detection of small molecule biotoxins is almost blank

Method used

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  • Kit and method for quantitative determination of ochratoxin A content in food product

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Embodiment Construction

[0023] The present invention is further described in detail by the following examples, which are illustrative, not restrictive, and the protection scope of the present invention cannot be limited by the following examples.

[0024] A qualitative detection kit for the content of ochratoxin A in food, consisting of a test strip and a reagent group, wherein the test strip is coated with a linker between ochratoxin A and ovalbumin as the detection area A of the test strip And the goat anti-rabbit secondary antibody was used as the solid phase carrier of the validity standard area B of the test strip. The solid phase carrier was a nitrocellulose membrane, and the spotting amount of ochratoxin A and ovalbumin conjugate was 1 μg. The size of the paper strip is 1.0×4.5cm, the widths of the two areas A and B are equal, 5mm, and the distance between the two areas A and B is 10cm.

[0025] The specific operation is as follows:

[0026] The OTA-OVA linker was spotted on a nitrocellulose ...

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Abstract

The invention belongs to the immunity analysi technical field, in particular to a reagent box for qualitatively detecting the content of ochratoxin A in food and the detecting method thereof. The reagent box comprises a test paper strip and reagent, wherein, the test paper strip is of a solid support which takes a joining enveloped with the ochratoxin A and egg-albumin as a detecting area A of the test pater, and takes a goat anti-rabbit secondary antibody as an effective standard area B of the test paper strip. The invention has the advantages of simple operation, high detecting sensitivity, strong specificity, good veracity, low detecting cost, better stability, etc., in addition, the range of application is wider. The invention is mainly used for the qualitative detection of the content of the ochratoxin A in the food and the provision.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, in particular to a qualitative detection kit for the content of ochratoxin A in food and a detection method thereof. Background technique [0002] Ochratoxin is a toxic metabolite produced by certain toxin-producing strains of Ochraus and Penicillium after infecting grain, food, feed and other agricultural and sideline products. Ochratoxin A is the most toxic and the highest yield of ochratoxins. It has a strong toxic effect on humans and animals, and can cause kidney atrophy, fetal malformation, abortion, and death in animals, and is highly carcinogenic. OTA is found in most grains, mainly including barley, wheat, oats, corn and coffee. At the same time, poultry fed on these grains can become contaminated. So ochratoxin is another toxin that has attracted worldwide attention after aflatoxin. In 1993, The International Agency for Research on Cancer classified ochratoxin as a Group 2B c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N1/34G01N21/31
Inventor 王硕王向红郑文杰张燕
Owner TIANJIN UNIV OF SCI & TECH
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