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Detoxifying fast breeding technique for edible lily

A technology for eating lilies and lilies, applied in applications, plant cells, horticulture, etc., can solve the problems of affecting economic value, susceptibility to viruses, low reproduction coefficient, etc.

Inactive Publication Date: 2011-11-16
上海光兆植物速生技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional lily propagation method is small bulb propagation, which has a low reproduction coefficient and is susceptible to viruses, which affects its economic value.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A virus-free and rapid propagation technology for edible lily, which includes in sequence: taking edible lily micro-shoot tips, starting, and substituting strong seedlings to obtain virus-free lily tissue-cultured seedlings, and inducing regeneration of scales through strong seedlings , the regenerated scales induce clustered buds, the clustered buds are subcultured, and then the regenerated scales are induced after the strong seedlings, and the regenerated scales are then induced to induce adventitious buds, and the cycle is alternated to carry out batch propagation, wherein the virus-free lily group Seedling cultivation comprises the following steps:

[0026] The first step is to take edible lily bulbs and undergo sand culture for 7-10 days, peel off the outer scales, clean and disinfect, peel off the growth points under the dissecting microscope, and cut off the stem tips with a size of 0.1-0.2mm;

[0027] In the second step, the micro-shoot tips were inoculated and ...

Embodiment 2

[0032] A technology for detoxification and rapid propagation of edible lily. On the basis of Example 1, regenerating scales to induce adventitious buds, including in sequence: taking edible lily micro shoot tips, starting and substituting strong seedlings to obtain detoxified lily tissue culture After the seedlings are tested as virus-free, the regenerated scales are induced through the strong seedlings, and the regenerated scales are used to induce adventitious buds. Circulation, carry out batch propagation, wherein, described detoxification lily tissue culture seedling cultivation step comprises:

[0033] The first step is to take edible lily bulbs and undergo sand culture for 7-10 days, peel off the outer scales, clean and disinfect, peel off the growth point under the dissecting microscope, and cut off the stem tips with a size of 0.1-0.2mm as the starting point for propagation Material;

[0034] In the second step, the micro-shoot tips were inoculated on the starting med...

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PUM

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Abstract

The invention relates to a virus elimination and rapid propagation technique for edible lily. The technique comprises the following processes in sequence: edible lily stem tip is taken to obtain virus elimination lily tissue culture seedling by starting and subculturing strong seedling; strong seedling induces regeneration lepisphere; regeneration lepisphere induces multiple shoot or adventitious bud; the multiple shoot or the adventitious bud induces regeneration lepisphere after subculturing of strong seedling and then the regeneration lepisphere induces the multiple shoot or the adventitious bud again. Batch expanding propagation is carried out through the cyclic alternating. The culture of tissue culture seedling of the virus elimination lily comprises the following steps: edible lilybulb is sand-cultured for 7-10 days; the outer scale is stripped and then the stripped lily bulb is cleaned and sterilized; growing points are peeled under an anatomical lens to get stem tip of 0.1-0.2mm as initial material of expanding propagation; the micro stem tip is inoculated on an initial medium of MS+6BA 0.5-2.5mg / L+NAA 0.1-0.5mg / L to be cultured; the tissue culture seedling of the virus elimination lily with non virus ratio of 100 percent through virus detection is obtained after 3-4 days of dark culture and then light culture. Industrial tissue culture and rapid propagation can be realized.

Description

technical field [0001] The invention relates to a detoxification and rapid propagation technology for edible lily, in particular to a tissue culture and rapid propagation technology with high detoxification efficiency and rapid propagation efficiency for Yixing lily. Background technique [0002] Lily virus disease is widely distributed and harmful. It is one of the main diseases in the production of edible lily and fresh-cut flower production, and has been paid attention to all over the world. In recent years, with the rapid increase in the number of lily species introduced and the planting area in my country, as well as the self-propagation and self-use of non-standard bulbs, viral diseases have become prevalent in various lily planting areas in my country, with a general incidence rate of 40-50%. The poisonous rate is more than 90%. After the lily is infected with the virus, it will carry the virus for life and suffer for a long time, destroying the normal physiological f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 宋学孟陈丽张承妹章振华唐寅陈明亮
Owner 上海光兆植物速生技术有限公司