CA15-3, CEA, CA19-9, CA12-5, SF mammary cancer colloidal gold five joint inspection diagnostic reagent kit
A diagnostic kit, breast cancer technology, applied in the field of biomedicine, can solve the problems of difficulty in detecting early or recurrent breast cancer patients, inability to provide, low detection sensitivity and specificity, etc.
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[0030] Example 1 Preparation of the colloidal gold five-inspection diagnostic kit of the present invention
[0031] 1. Preparation of monoclonal antibodies against CA15-3, CEA, CA19-9, CA12-5, SF
[0032] CA15-3, CEA, CA19-9, CA12-5, SF (CEA antibody purchased from Tianjin Tianjian Biopharmaceutical Co., Ltd., SF antibody purchased from Medix Biochemica, CA125, CA153, CA199 purchased from CanAg Diagnostics AB) / c Pure line mice are immunized, and when the mouse serum produces the corresponding antibody, the spleen cells can be fused with SP2 / 0 cells. Hybridoma cells were selected by HAT, and McAb was detected by enzyme-linked immunosorbent assay (ELISA). For hybridomas with positive detection antibodies, the limiting dilution method should be used for cloning, while the positive cell lines should be preserved, and a large number of monoclonal antibodies can be prepared using BALB / c pure-line mice. At the same time, antibody specific identification, identification of epitope recogn...
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